Comparative Transcriptome Analysis Reveals Putative Genes Responsible for High Theacrine Content in Kucha (Camellia kucha (Chang et Wang) Chang)
文献类型： 外文期刊
作者： Li, Hongjian 1 ; Fang, Kaixing 1 ; Qin, Dandan 1 ; Jiang, Xiaohui 1 ; Wang, Qiushuang 1 ; Pan, Chendong 1 ; Li, Bo 1 ; Wa 1 ;
作者机构： 1.Guangdong Acad Agr Sci, Tea Res Inst, Guangdong Key Lab Tea Plant Resources Innovat & U, Guangzhou 510640, Guangdong, Peoples R China
关键词： Tea plant; Transcriptome; Theacrine; Biosynthesis
期刊名称：TROPICAL PLANT BIOLOGY （ 影响因子：1.512； 五年影响因子：2.011 ）
ISSN： 1935-9756
年卷期： 2021 年 14 卷 1 期
页码：
收录情况： SCI
摘要： The purine alkaloid theacrine, synthesized in a high amount in Kucha (Camellia kucha (Chang et Wang) Chang), has shown multiple pharmacological effects such as anti-depression, dehydration and hypnotic activities. It is critical to better understand the theacrine metabolism at the molecular level for future breeding programs. To identify the putative genes involved in theacrine biosynthesis, we carried out comparative transcriptome analysis between Kucha (Kucha-6 and Kucha-11) and conventional varieties (Yinghong 9 and Qingxin 1). HPLC showed that Kucha synthesized more than 27-fold theacrine in comparison to conventional varieties. A total of similar to 671.61 million high quality clean reads were yielded, and 71.42 similar to 76.87% of these were aligned to the reference genome for each library. We identified 19,095 differentially expressed genes (DEGs) by a pairwise approach, among which 1,142 common DEGs between Kucha and conventional varieties were screened. Functional annotation allowed the identified of 20 oxidoreductase (OR) genes and seven methyltransferase (MT) genes with enhanced expression in Kucha, which might be associated with theacrine diversity in the examined materials. Particularly, two N-methyltransferase-encoding genes (TEA010054 and TEA022559) may catalyze the final methylation step during theacrine synthesis in Kucha. The information generated will be helpful in identifying candidate genes and breeding tea plant with high theacrine content.
- 相关文献
作者其他论文 更多>>
-
Interferon regulatory factors inhibit TiLV replication by activating interferon-a3 in tilapia (Oreochromis niloticus)
作者：Ke, Zishan;Wen, Jing;Wang, Yingying;Li, Bo;Zhang, Defeng;Mo, Xubing;Li, Yingying;Ren, Yan;Yin, Jiyuan;Shi, Cunbin;Wang, Qing;Zheng, Shucheng;Ke, Zishan;Li, Bo;Zheng, Shucheng;Wu, Siyu;Zheng, Shucheng
关键词：Interferon regulatory factor; Interferon; Innate immunity; Tilapia; Tilapia lake virus
-
Identification of key volatile and odor-active compounds in 10 main fragrance types of Fenghuang Dancong tea using HS-SPME/GC-MS combined with multivariate analysis
作者：Qin, Dandan;Wang, Qiushuang;Jiang, Xiaohui;Ni, Erdong;Fang, Kaixing;Li, Hongjian;Wang, Qing;Pan, Chendong;Li, Bo;Wu, Hualing
关键词：Oolong tea; Fenghuang Dancong tea; Volatile compound; Fragrance type; Odor activity value; Floral odor; Partial least squares -discriminant analysis
-
Characterization of the Aroma Profiles of Guangdong Black Teas Using Non-Targeted Metabolomics
作者：Wang, Qiushuang;Qin, Dandan;Jiang, Xiaohui;Fang, Kaixing;Li, Bo;Wang, Qing;Pan, Chendong;Ni, Erdong;Li, Hongjian;Chen, Dong;Wu, Hualing
关键词：black teas; volatile flavor compounds; metabolomics
-
Gene mining and genomics-assisted breeding empowered by the pangenome of tea plant Camellia sinensis
作者：Chen, Shuai;Wang, Pengjie;Kong, Weilong;Zhang, Shengcheng;Yu, Jiaxin;Wang, Yibin;Chen, Xiao;Gao, Yingying;Qu, Shenyang;Wang, Fang;Zhang, Qing;Zhang, Xingtan;Chai, Kun;Jiang, Mengwei;Lei, Wenlong;Wang, Wenling;Wang, Yinghao;Gu, Mengya;Sun, Weijiang;Ye, Naixing;Fang, Kaixing;Wu, Hualing;Ma, Chunlei
关键词：
-
Color Shade Nets Affect Plant Growth and Seasonal Leaf Quality of Camellia sinensis Grown in Mississippi, the United States
作者：Zhang, Qianwen;Bi, Guihong;Li, Tongyin;Xing, Zhiheng;LeCompte, Judson;Harkess, Richard L.;Wang, Qiushuang
关键词：tea; cold damage; polyphenols; carbohydrates; caffeine; catechins; protected culture
-
Understanding carotenoid biosynthetic pathway control points using metabolomic analysis and natural genetic variation
作者：Wen, Weiwei;Jia, Xinxin;Zhang, Weiyi;Jiang, Xiaohui;Jiang, Xiaohui;Jiang, Xiaohui;Fernie, Alisdair R.
关键词：
-
Characterization of Effects of Different Tea Harvesting Seasons on Quality Components, Color and Sensory Quality of "Yinghong 9" and "Huangyu" Large-Leaf-Variety Black Tea
作者：Ye, Fei;Li, Bo;Chen, Haiqiang;Qiao, Xiaoyan;Ye, Fei;Guo, Xinbo
关键词：harvesting seasons; large leaf variety black tea; catechins; caffeine; color; physicochemical qualities
