您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Molecular characterization and expression analysis of Tf_TLR4 and Tf_TRIL in yellowcatfish Tachysurus fulvidraco responding to Edwardsiella ictaluri challenge

文献类型： 外文期刊

作者： Chen, Kaiwei 1 ; Zhao, Feng 1 ; Ouyang, Gang 2 ; Shi, Zechao 3 ; Ma, Lina 1 ; Wang, Bingchao 1 ; Guo, Ronghuan 1 ; Xiao, 1 ;

作者机构： 1.Huazhong Agr Univ, Minist Agr & Rural Affair, Key Lab Agr Anim Genet Breeding & Reprod,Key Lab, Dept Aquat Anim Med,Coll Fisheries,Minist Educ, Wuhan 430070, Hubei, Peoples R China

2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China

3.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan 430223, Peoples R China

4.Huazhong Agr Univ, Coll Fisheries, Hubei Engn Technol Res Ctr Aquat Anim Dis Control, Wuhan 430070, Peoples R China

关键词： Tachysurus fulvidraco; TLR4; TRIL

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：6.953； 五年影响因子：6.737 ）

ISSN： 0141-8130

年卷期： 2021 年 167 卷

页码：

收录情况： SCI

摘要： Toll-like receptors play significant roles in defensing against pathogen invasion. In this study, TLR4 and TRIL from Yellow catfish Tachysurus fulvidraco (Tf), were identified and characterized. The open reading frames of the Tf_TLR4 and Tf_TRIL genes were 2466 bp and 1827 bp in length, encoding 821 and 608 amino acids, respectively. The Tf_TLR4 consists of LRRs, a transmembrane domain and a TIR domain, and Tf_TRIL only contains LRRs and TIR domain. Homologous identity revealed that both Tf_TLR4 and Tf_TRIL have high protein sequence similarity with that of channel catfish Ictalurus punctatus. Both the Tf_TLR4 and Tf_TRIL genes were highly expressed in head kidney and brain, respectively. The mRNA expression levels of Tf_TLR4 and Tf_TRIL genes were up-regulated in intestine and immune-related tissues after challenge of Edwardsiella ictaluri. The microscopic observation of the gut showed that the pathological changes in midgut and hindgut are more obvious than that in foregut after challenged with E. ictaluri. These results indicate that these two genes play potential roles in the host defense against E. ictaluri invasion. This study will provide valuable information to better understand the synergistic roles of TLR4 and TRIL in the innate immune system of yellow catfish and other fish. (C) 2020 Elsevier B.V. All rights reserved.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台