Ultrasound-assisted dispersive solid-phase extraction combined with reversed-phase high-performance liquid chromatography-photodiode array detection for the determination of nine biogenic amines in canned seafood
文献类型： 外文期刊
作者： Wang, Jingyu 1 ; Liu, Zhidong 2 ; Qu, Yinghong 1 ;
作者机构： 1.Shanghai Ocean Univ, Coll Food Sci & Technol, Shanghai Engn Res Ctr Aquat Prod Proc & Preservat, Shanghai 200120, Peoples R China
2.Chinese Acad Fishery Sci, East China Sea Fishery Res Inst, Key Lab Ocean & Polar Fisheries, Minist Agr & Rural Affair, Shanghai 200090, Peoples R China
关键词： Biogenic amines; Canned seafood; HPLC; Ultrasound-assisted dispersive solid-phase extraction
期刊名称：JOURNAL OF CHROMATOGRAPHY A （ 影响因子：4.759； 五年影响因子：4.302 ）
ISSN： 0021-9673
年卷期： 2021 年 1636 卷
页码：
收录情况： SCI
摘要： This work describes an ultrasound-assisted dispersive solid-phase extraction method combined with reversed-phase high-performance liquid chromatography-photodiode array detection (UADSPE-RPLC-PDA) for the determination of nine common biogenic amines (BAs) in canned seafood. The pretreatment extraction solvent, ultrasonic treatment duration, and derivatization conditions were optimized. The method was validated on the basis of the limit of detection (LOD), limit of quantification (LOQ), precision, and accuracy and successfully applied to analyze four canned fish, two canned shrimp and four canned shellfish samples. LODs of 0.08-0.25 mg kg(-1) were achieved, and the correlation coefficient of determination was 0.9994-0.9997. The method had high precision and accuracy, with relative standard deviations (RSDs) and recoveries of 0.44 to 6.83% and 72.57 to 99.74%, respectively, suggesting the effectiveness of ultrasound-assisted extraction at increasing the solubility of the target analytes in the solvent system and the feasibility of UADSPE-RPLC-PDA for determining trace BAs in canned seafood. (C) 2020 Elsevier B.V. All rights reserved.
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