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Forkhead transcription factor O1 (FoxO1) in torafugu pufferfish Takifugu rubripes: Molecular cloning, in vitro DNA binding, and target gene screening in fish metagenome

文献类型： 外文期刊

作者： Reyes, Anthony Canela 1 ; Egwu, Elvis 1 ; Yu, Ermeng 1 ; Sanchez, Ashley N. 1 ; De La O, Linda 1 ; Elijah, Osasenag 1 ;

作者机构： 1.Univ Houston, Sch Arts & Sci, Victoria, TX 77901 USA

2.Pearl River Fisheries Res Inst CAFS, Key Lab Trop & Subtrop Fishery Resource Applicat, Xingyu Rd 1, Guangzhou 510380, Peoples R China

3.Xinxiang Med Univ, Sch Basic Med Sci, Xinxiang 453003, Henan, Peoples R China

4.Northwest A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Shaanxi, Peoples R China

关键词： Carbohydrate metabolism; DAF16-binding element; Fish metagenome; FoxO1; In silico screening; insulin/IGF pathway; Insulin-responsive element; Takifugu rubripes; Torafugu

期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）

ISSN： 0378-1119

年卷期： 2021 年 768 卷

页码：

收录情况： SCI

摘要： The fish insulin/insulin-like growth factor (IGF) pathway has weak control over carbohydrate metabolism. To understand the molecular basis for the metabolic diversity, we characterized the forkhead box transcription factor O1A (FoxO1A), a downstream target of the insulin/IGF pathway, in torafugu Takifugu rubripes. The cloned torafugu FoxO1A cDNA contained all conserved features critical for its transcriptional activity and a unique unspliced intron encoding a poly-glutamine stretch. Torafugu FoxO1A showed the IGF-dependent nuclear exclusion and in vitro binding to the well-conserved FoxO1 binding site, DAF-16 binding element (DBE), but failed to bind to the insulin-responsive element by which mammalian FoxO1 mediates insulin effects. The subsequent in silico genomic screening provided a list of 587 potential torafugu FoxO1A target genes containing the DBE. Some carbohydrate metabolic genes regulated by FoxO1 in mammals were not included in the list. We further identified about 250 potential fish FoxO1 target genes by integrating results of the DBE screening against fish metagenome that contained 262 species. Neuronal processes appeared to be the common major function of fish FoxO1, although further annotation of the potential target genes is required. These results provide a part of the molecular basis underlying the weak association between the insulin/IGF pathway and carbohydrate metabolism in fish.

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