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Influence of N-acetylcysteine on steroidogenesis and gene expression in porcine placental trophoblast cells

文献类型： 外文期刊

作者： Ding, Hongxiang 1 ; Yang, Yuze 4 ; Wei, Shangli 1 ; Spicer, Leon J. 2 ; Kenez, Akos 6 ; Xu, Wei 6 ; Liu, Yan 1 ; Feng, Ta 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci BAAFS, Inst Anim Husb & Vet Med IAHVM, Beijing 100097, Peoples R China

2.Oklahoma State Univ, Joint Lab Anim Sci IAHVM BAAFS, Beijing 100097, Peoples R China

3.Oklahoma State Univ, Div Agr Sci & Nat Resource, Beijing 100097, Peoples R China

4.Beijing Gen Stn Anim Husb, Beijing 100107, Peoples R China

5.Oklahoma State Univ, Dept Anim & Food Sci, Stillwater, OK 74078 USA

6.City Univ Hong Kong, Dept Infect Dis & Publ Hlth, Hong Kong, Peoples R China

关键词： N-acetylcysteine; Trophoblast; Cell differentiation; Steroidogenesis; Sow

期刊名称：THERIOGENOLOGY （ 影响因子：2.74； 五年影响因子：2.93 ）

ISSN： 0093-691X

年卷期： 2021 年 161 卷

页码：

收录情况： SCI

摘要： N-acetylcysteine (NAC) is a widely used anti-inflammatory agent and antioxidant in vivo and in vitro. As a nutritional supplement, NAC can improve production and reproductive performances in animals through enhancing placental function and regulating hormone production. Trophoblast proliferation and steroid hormone production are two major functions in the placenta. We hypothesized that the effects of NAC on placental function is due to its direct and indirect effects on gene expression in placental trophoblast cells (pTr). To evaluate this hypothesis, we investigated the effects of NAC on steroidogenesis, gene expression, and cell proliferation in porcine pTr in vitro. pTr were treated with NAC in serum-free medium for 24 h with different concentrations (0, 0.1 mu M,1.0 mu M,10.0 mu M, 0.1 mM, 1.0 mM, and 10.0 mM). Low-dose NAC (1 tiM) stimulated pTr proliferation and decreased progesterone production, while increasing estradiol production (P < 0.05). High-dose NAC (10 mM) suppressed cell proliferation (P < 0.05), but had no effect on steroidogenesis. Low-dose NAC increased CCDN1 and decreased CASP3 and CASP8 mRNA levels (P < 0.05), whereas high-dose NAC decreased CDK4 and CCDN1 and increased CASP3 mRNA levels (P < 0.05). NAC had no effect on the mRNA abundance of StAR and HSD3B. Low-dose NAC upregulated CYP19A1 mRNA expression, and high-dose NAC downregulated CYP11A1 mRNA abundance (P < 0.05). Only low-dose NAC increased NOS3 mRNA abundance and tetrahydrobiopterin reduction (BH4/BH2 ratio). We conclude that NAC may act directly and indirectly on pTr with a dose-dependent manner and may regulate placental function by affecting pTr differentiation via regulating pTr steroid synthesis, cell proliferation, and apoptosis in sows. (C) 2020 Elsevier Inc. All rights reserved.

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