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Label-Free Amperometric Immunosensor Based on Versatile Carbon Nanofibers Network Coupled with Au Nanoparticles for Aflatoxin B-1 Detection

文献类型： 外文期刊

作者： Huang, Yunhong 1 ; Zhu, Fei 2 ; Guan, Jinhua 2 ; Wei, Wei 1 ; Zou, Long 1 ;

作者机构： 1.Zhejiang Acad Agr Sci, Inst Agroprod Safety & Nutr, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou 310021, Peoples R China

2.Jiangxi Normal Univ, Coll Life Sci, Nanchang Key Lab Microbial Resources Exploitat &, Nanchang 330022, Jiangxi, Peoples R China

关键词： aflatoxin B-1; immunosensor; carbon nanofibers; Au nanoparticles; electrochemical detection

期刊名称：BIOSENSORS-BASEL （ 影响因子：5.519； 五年影响因子：5.313 ）

ISSN：

年卷期： 2021 年 11 卷 1 期

页码：

收录情况： SCI

摘要： Facile detection methods for mycotoxins with high sensitivity are of great significance to prevent potential harm to humans. Herein, a label-free amperometric immunosensor based on a 3-D interconnected carbon nanofibers (CNFs) network coupled with well-dispersed Au nanoparticles (AuNPs) is proposed for the quantitative determination of aflatoxin B-1 (AFB(1)) in wheat samples. In comparison to common carbon nanotubes (CNTs), the CNFs network derived from bacterial cellulose biomass possesses a unique hierarchically porous structure for fast electrolyte diffusion and a larger electrochemical active area, which increases the peak current of differential pulse voltammetry curves for an immunosensor. Combined with AuNPs that are incorporated into CNFs by using linear polyethyleneimine (PEI) as a soft template, the developed Au@PEI@CNFs-based immunosensor showed a good linear response to AFB(1) concentrations in a wide range from 0.05 to 25 ng mL(-1). The limit of detection was 0.027 ng mL(-1) (S/N = 3), more than three-fold lower than that of an Au@PEI@CNTs-based sensor. The reproducibility, storage stability and selectivity of the immunosensor were proved to be satisfactory. The developed immunosensor with appropriate sensitivity and reliable accuracy can be used for the analysis of wheat samples.

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