OsCER1 regulates humidity-sensitive genic male sterility through very-long-chain (VLC) alkane metabolism of tryphine in rice
文献类型： 外文期刊
作者： Ni, Erdong 1 ; Deng, Li 1 ; Chen, Huiqiong 1 ; Lin, Jianwen 1 ; Ruan, Jiamin 1 ; Liu, Zhenlan 1 ; Zhuang, Chuxiong 1 ; Zh 1 ;
作者机构： 1.South China Agr Univ, Coll Life Sci, State Key Lab Conservat & Utilisat Subtrop Agrobi, Guangzhou 510642, Peoples R China
2.Guangdong Lab Lingnan Modern Agr, Guangzhou 510642, Guangdong, Peoples R China
3.Guangdong Acad Agr Sci, Tea Res Inst, Guangzhou 510640, Guangdong, Peoples R China
4.Guangdong Key Lab Tea Plant Resources Innovat & U, Guangzhou 510640, Guangdong, Peoples R China
关键词： environment-sensitive male sterility; humidity; very-long-chain (VLC) alkanes; OsCER1
期刊名称：FUNCTIONAL PLANT BIOLOGY （ 影响因子：3.101； 五年影响因子：3.248 ）
ISSN： 1445-4408
年卷期： 2021 年 48 卷 5 期
页码：
收录情况： SCI
摘要： Humidity-sensitive genic male sterility (HGMS) is a novel type of environment-sensitive male sterility (EGMS) which plants are male sterile at low humidity and male fertile at high humidity. Previous studies have revealed that OsCER1 contributes to very-long-chain (VLC) alkanes biosynthesis in rice (Oryza sativa L.). Here, applying the CRISPR/Cas9 technique, we obtained two independent OsCER1 knockout lines (OsCER1Cas). Both OsCER1Cas lines exhibited HGMS. Mutant pollen showed defects in adhesion and germination on stigmas at low humidity, whereas high humidity enhanced the pollen germination rate. Transmission electron microscopy (TEM) observations of mutant pollen revealed abnormal tryphine structure, potentially representing the basis of HGMS. Furthermore, co-pollination with mixed OsCER1Cas mutant and maize (Zea mays L.) pollen could rescue the fertility of the mutant, thereby establishing the key role of tryphine in germination on stigmas. OsCER1 knockout might affect VLC alkane metabolism and therefore alter the lipid composition of tryphine. It could lead to the defects in pollen grain adhesion, hydration and germination, resulting in HGMS. This work identified the mechanism of HGMS induced by VLC alkanes in rice and the generality of tryphine in different species of Gramineae.
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