OsCER1 regulates humidity-sensitive genic male sterility through very-long-chain (VLC) alkane metabolism of tryphine in rice
文献类型： 外文期刊
作者： Ni, Erdong 1 ; Deng, Li 1 ; Chen, Huiqiong 1 ; Lin, Jianwen 1 ; Ruan, Jiamin 1 ; Liu, Zhenlan 1 ; Zhuang, Chuxiong 1 ; Zh 1 ;
作者机构： 1.South China Agr Univ, Coll Life Sci, State Key Lab Conservat & Utilisat Subtrop Agrobi, Guangzhou 510642, Peoples R China
2.Guangdong Lab Lingnan Modern Agr, Guangzhou 510642, Guangdong, Peoples R China
3.Guangdong Acad Agr Sci, Tea Res Inst, Guangzhou 510640, Guangdong, Peoples R China
4.Guangdong Key Lab Tea Plant Resources Innovat & U, Guangzhou 510640, Guangdong, Peoples R China
关键词： environment-sensitive male sterility; humidity; very-long-chain (VLC) alkanes; OsCER1
期刊名称：FUNCTIONAL PLANT BIOLOGY （ 影响因子：3.101； 五年影响因子：3.248 ）
ISSN： 1445-4408
年卷期： 2021 年 48 卷 5 期
页码：
收录情况： SCI
摘要： Humidity-sensitive genic male sterility (HGMS) is a novel type of environment-sensitive male sterility (EGMS) which plants are male sterile at low humidity and male fertile at high humidity. Previous studies have revealed that OsCER1 contributes to very-long-chain (VLC) alkanes biosynthesis in rice (Oryza sativa L.). Here, applying the CRISPR/Cas9 technique, we obtained two independent OsCER1 knockout lines (OsCER1Cas). Both OsCER1Cas lines exhibited HGMS. Mutant pollen showed defects in adhesion and germination on stigmas at low humidity, whereas high humidity enhanced the pollen germination rate. Transmission electron microscopy (TEM) observations of mutant pollen revealed abnormal tryphine structure, potentially representing the basis of HGMS. Furthermore, co-pollination with mixed OsCER1Cas mutant and maize (Zea mays L.) pollen could rescue the fertility of the mutant, thereby establishing the key role of tryphine in germination on stigmas. OsCER1 knockout might affect VLC alkane metabolism and therefore alter the lipid composition of tryphine. It could lead to the defects in pollen grain adhesion, hydration and germination, resulting in HGMS. This work identified the mechanism of HGMS induced by VLC alkanes in rice and the generality of tryphine in different species of Gramineae.
- 相关文献
作者其他论文 更多>>
-
Controlling diurnal flower-opening time by manipulating the jasmonate pathway accelerates development of indica-japonica hybrid rice breeding
作者：Wang, Mumei;Zhu, Xiaopei;Huang, Zhen;Chen, Minghao;Xu, Peng;Liao, Shitang;Zhao, Yongzhen;Gao, Yannan;He, Jiahui;Luo, Yutong;Chen, Huixuan;Wei, Xiaoying;Zhu, Liya;Zhuang, Chuxiong;Liu, Zhenlan;Zhou, Hai;Wang, Mumei;Nie, Shuai;Dong, Jingfang;Zhao, Junliang;Nie, Shuai;Dong, Jingfang;Zhao, Junliang;Nie, Shuai;Dong, Jingfang;Zhao, Junliang
关键词：rice; hybrid breeding; yield of hybrid seed production; diurnal flower-opening time; jasmonate
-
The OsMYC2-JA feedback loop regulates diurnal flower-opening time via cell wall loosening in rice
作者：Zhu, Xiaopei;Wang, Mumei;Huang, Zhen;Chen, Minghao;Xu, Peng;Liao, Shitang;Gao, Yannan;Zhao, Yongzhen;Chen, Huixuan;He, Jiahui;Luo, Yutong;Wei, Xiaoying;Zhuang, Chuxiong;Liu, Zhenlan;Zhou, Hai;Wang, Mumei;Zhu, Liya;Liu, Chuanhe;Huang, Jilei;Zhao, Xinhui;Zhao, Junliang;Zhang, Zemin
关键词：rice; hybrid breeding; diurnal flower-opening time; jasmonate; OsMYC2
-
BOTRYOID POLLEN 1 regulates ROS-triggered PCD and pollen wall development by controlling UDP-sugar homeostasis in rice
作者：Chen, Huiqiong;Zhang, Shuqing;Li, Ruiqi;Peng, Guoqing;Chen, Weipan;Liu, Minglong;Zhu, Liya;Xiao, Yueping;Ni, Jinlong;Liu, Zhenlan;Zhuang, Chuxiong;Xie, Yongyao;Chu, Zhizhan;Zhou, Hai;Chen, Huiqiong;Rautengarten, Carsten;Heazlewood, Joshua L.;Song, Fengshun;Ni, Jinlong;Wu, Aimin
关键词：
-
Identification of key volatile and odor-active compounds in 10 main fragrance types of Fenghuang Dancong tea using HS-SPME/GC-MS combined with multivariate analysis
作者：Qin, Dandan;Wang, Qiushuang;Jiang, Xiaohui;Ni, Erdong;Fang, Kaixing;Li, Hongjian;Wang, Qing;Pan, Chendong;Li, Bo;Wu, Hualing
关键词：Oolong tea; Fenghuang Dancong tea; Volatile compound; Fragrance type; Odor activity value; Floral odor; Partial least squares -discriminant analysis
-
The E3 ubiquitin ligase CSIT1 regulates critical sterility-inducing temperature by ribosome- associated quality control to safeguard two-line hybrid breeding in rice
作者：Peng, Guoqing;Liu, Minglong;Zhu, Liya;Wang, Qinghua;Wang, Mumei;Chen, Huiqiong;Xiao, Yueping;Zhang, Yongjie;Hong, Haona;Liu, Zhenlan;Wang, Yingxiang;Zhuang, Chuxiong;Zhou, Hai;Peng, Guoqing;Zhou, Lingyan;Luo, Wenlong;Luo, Ziliang;Guo, Guoqiang
关键词：hybrid breeding; thermo-sensitive genic male sterility; critical sterility-inducing temperature; ribo-some-associated quality control; programmed cell death
-
Characterization of the Aroma Profiles of Guangdong Black Teas Using Non-Targeted Metabolomics
作者：Wang, Qiushuang;Qin, Dandan;Jiang, Xiaohui;Fang, Kaixing;Li, Bo;Wang, Qing;Pan, Chendong;Ni, Erdong;Li, Hongjian;Chen, Dong;Wu, Hualing
关键词：black teas; volatile flavor compounds; metabolomics
-
A cytosolic pentatricopeptide repeat protein is essential for tapetal plastid development by regulating OsGLK1 transcript levels in rice
作者：Zheng, Shaoyan;Lu, Jingqin;Li, Jing;Jiang, Dagang;Ye, Simiao;Bu, Wenli;Liu, Zhenlan;Zhou, Hai;Zhuang, Chuxiong;Zheng, Shaoyan;Lu, Jingqin;Li, Jing;Jiang, Dagang;Ye, Simiao;Bu, Wenli;Liu, Zhenlan;Zhou, Hai;Zhuang, Chuxiong;Dong, Jingfang;Yu, Haopeng;Ding, Yiliang
关键词：cytoplasm-localized; OsGLK1; post-transcriptional regulation; PPR; programmed cell death; rice (Oryza sativa); tapetal plastid
