Efficient direct preparation of antifungal Alteramide B from Lysobacter enzymogenes fermentation broth by macroporous resin adsorption
文献类型： 外文期刊
作者： Tang, Bao 1 ; Chen, Xian 1 ; Laborda, Pedro 2 ; Liu, Fengquan 1 ;
作者机构： 1.Jiangsu Acad Agr Sci, Inst Plant Protect, Jiangsu Key Lab Food Qual & Safety, State Key Lab Cultivat Base,Minist Sci & Technol, Nanjing 210014, Peoples R China
2.Nantong Univ, Sch Life Sci, Nantong 226019, Peoples R China
关键词： Alteramide B; Lysobacter enzymogenes; Macroporous adsorption resins; Biological pesticide
期刊名称：BIORESOURCE TECHNOLOGY （ 影响因子：9.642； 五年影响因子：9.237 ）
ISSN： 0960-8524
年卷期： 2021 年 319 卷
页码：
收录情况： SCI
摘要： Alteramide B (ATB) is an antifungal metabolite produced by Lysobacter enzymogenes. However, its separation method has not been explored. This study attempted to directly adsorb ATB from fermentation broth using macroporous adsorption resins (MARs) NKA resin exhibited better adsorption as well as desorption capacities. The static and dynamic adsorption characteristics were assessed to determine the following optimal separation conditions: initial fermentation broth with a pH of 12.0, 2 BV/h flow rate, 8 BV loading volume, and 6 BV 80% aqueous ethanol for elution. After a single treatment, ATB content in the final product was higher by 4.51-fold (i.e, from 12.72 +/- 1.21% to 57.35 +/- 3.46%), resulting in a recovery yield of 86.20 +/- 4.47%. In addition, NKA resin showed superior reusability within eight cycles of adsorption/desorption. The developed method is thus a simple, efficient, and economical process for ATB separation.
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