Knockdown of igf3 gene by RNA interference causes gonadal development inhibition of common carp (Cyprinus carpio)
文献类型： 外文期刊
作者： Song, Feibiao 1 ; Wang, Lanmei 2 ; Yu, Zhenzhen 3 ; Zhu, Wenbin 2 ; Fu, Jianjun 2 ; Dong, Zaijie 1 ;
作者机构： 1.Nanjing Agr Univ, Wuxi Fisheries Coll, Wuxi, Jiangsu, Peoples R China
2.Chinese Acad Fishery Sci, Key Lab Freshwater Fisheries & Germplasm Resource, Minist Agr, Freshwater Fisheries Res Ctr, Wuxi, Jiangsu, Peoples R China
3.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai, Peoples R China
关键词： common carp; dsRNA; gonadal development; igf3; RNAi
期刊名称：AQUACULTURE RESEARCH （ 影响因子：2.082； 五年影响因子：2.415 ）
ISSN： 1355-557X
年卷期：
页码：
收录情况： SCI
摘要： RNA interference is an important method in studying the gene function of organisms. As a novel important gene in gonadal development of teleost fish, the function of igf3 is worth of researching. In this study, the igf3b-dsRNA of C. carpio was successfully synthesized, and long-term igf3 interference experiments were conducted. Intraperitoneal injection of igf3b-dsRNA could influence the serum sex hormone level and significantly reduced the expression level of insulin-like growth factors (IGFs) in hypothalamic-pituitary-gonad (HPG) axis. After a 60-day interference experiment, igf3 knockdown could affect the development of ovary and testis of juvenile C. carpio. These findings show that igf3 knockdown may have a negative effect on gonadal development of C. carpio through HPG axis.
- 相关文献
作者其他论文 更多>>
-
High-temperature stress response: Insights into the molecular regulation of American shad (Alosa sapidissima) using a multi-omics approach
作者：Luo, Mingkun;Zhu, Wenbin;Fu, Jianjun;Miao, Linghong;Dong, Zaijie;Liang, Zhengyuan;Li, Yulin;Liu, Ying;Shi, Xiulan;Dong, Zaijie;Feng, Bingbing;Xie, Xudong
关键词：Alosa sapidissima; Gills; Heat stress; Oxidative damage; Multi-omics
-
Effects of dietary supplementation of different ratios of koumine and gelsemin on the liver immunity of common carp (Cyprinus carpio, FFRC)
作者：Sun, Di;Ye, Bin;Wang, Dongjie;Zou, Jixing;Wang, Shaodan;Dong, Zaijie;Zou, Jixing;Dong, Zaijie
关键词：Alkaloid; Feed additive; Common carp; Cultivation
-
Genome-wide association study reveals candidate genes critical for skin pigmentation in common carp ( Cyprinus carpio ) strains including koi
作者：Shi, Xiulan;Luo, Mingkun;Dong, Zaijie;Zhu, Wenbin;Fu, Jianjun;Wang, Lanmei;Dong, Yalun;Luo, Mingkun;Dong, Zaijie;Guo, Jun;Lin, Kai
关键词：Common carp strains; Population differentiation; Linkage disequilibrium; Selected sweeping; Genome-wide association studies
-
Integrated transcriptome and microRNA analysis reveals molecular responses to high-temperature stress in the liver of American shad (Alosa sapidissima)
作者：Liu, Ying;Liang, Zhengyuan;Li, Yulin;Luo, Mingkun;Dong, Zaijie;Zhu, Wenbin;Fu, Jianjun;Luo, Mingkun;Dong, Zaijie;Feng, Bingbing;Xu, Wei;Wei, Panpan
关键词：Alosa sapidissima; Heat stress; Liver; Genes; miRNAs
-
Effect of dietary koumine on the immune and antioxidant status of carp (Cyprinus carpio) after Aeromonas hydrophila infection
作者：Wang, Qiujie;Sun, Di;Wang, Dongjie;Ye, Bin;Zhou, Aiguo;Zou, Jixing;Wang, Shaodan;Dong, Zaijie;Zou, Jixing;Dong, Zaijie
关键词：Koumine; Antioxidant capacity; Histopathology; Immune function
-
Effects of different combinations of koumine and gelsemine on growth performance, intestinal health, and transcriptome of Cyprinus carpio
作者：Ye, Bin;Wang, Qiujie;Wang, Dongjie;Zou, Jixing;Ye, Qiao;Wang, Zhenlu;Dong, Zaijie;Dong, Zaijie;Zou, Jixing
关键词：Alkaloid; Feed additive; Common carp; Cultivation
-
Single nucleotide polymorphism SNP19140160 A > C is a potential breeding locus for fast-growth largemouth bass (Micropterus salmoides)
作者：Hua, Jixiang;Zhong, Chunyi;Wang, Qingchun;Qiang, Jun;Hua, Jixiang;Zhong, Chunyi;Fu, Jianjun;Li, Yan;Tao, Yifan;Dong, Yalun;Lu, Siqi;Liu, Wenting;Qiang, Jun;Chen, Wenhua;Zhu, Geyan;Zhang, Maoyou;Wang, Jian
关键词：Largemouth bass; RAD-seq; SNP; Growth traits; Dominant genotypes
