文献类型： 外文期刊
作者： Chen, Shun-Fa 1 ; Liu, Xi-Chun 1 ; Xu, Jia-Kun 2 ; Li, Lianzhi 4 ; Lang, Jia-Jia 3 ; Wen, Ge-Bo 3 ; Lin, Ying-Wu 1 ;
作者机构： 1.Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China
2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Dev Polar Fisheries,Minist Ag, Lab Marine Drugs & Byprod,Pilot Natl Lab Marine S, Qingdao 266071, Peoples R China
3.Univ South China, Lab Prot Struct & Funct, Med Sch, Hengyang 421001, Peoples R China
4.Liaocheng Univ, Sch Chem & Chem Engn, Liaocheng 252059, Shandong, Peoples R China
期刊名称：INORGANIC CHEMISTRY （ 影响因子：5.165； 五年影响因子：4.815 ）
ISSN： 0020-1669
年卷期： 2021 年 60 卷 4 期
页码：
收录情况： SCI
摘要： Protein design has received much attention in the last decades. With an additional disulfide bond to enhance the protein stability, human A15C neuroglobin (Ngb) is an ideal protein scaffold for heme enzyme design. In this study, we rationally converted A15C Ngb into a multifunctional peroxidase by replacing the heme axial His64 with an Asp residue, where Asp64 and the native Lys67 at the heme distal site were proposed to act as an acid-base catalytic couple for H2O2 activation. Kinetic studies showed that the catalytic efficiency of A15C/H64D Ngb was much higher (similar to 50-80-fold) than that of native dehaloperoxidase, which even exceeds (similar to 3-fold) that of the most efficient native horseradish peroxidase. Moreover, the dye-decolorizing peroxidase activity was also comparable to that of some native enzymes. Electron paramagnetic resonance, molecular docking, and isothermal titration calorimetry studies provided valuable information for the substrate-protein interactions. Therefore, this study presents the rational design of an efficient multifunctional peroxidase based on Ngb with potential applications such as in bioremediation for environmental sustainabllity.
- 相关文献
作者其他论文 更多>>
-
Construction of artificial peroxidase based on myoglobin scaffold for efficient degradation of meloxicam
作者：Zhang, Weikang;Yang, Yadan;Wang, Fang;Xu, Jiakun;Zhang, Weikang;Yang, Yadan;Meng, Xiangmin;Lin, Ying-Wu
关键词：Meloxicam; Myoglobin; Biodegradation; Structure-function relationship; Degradation; Environmental remediation
-
Engineering Neuroglobin for Synthesis of Chiral Organoborons via Carbene B-H Insertion
作者：Sun, Li-Juan;Gao, Shu-Qin;Lin, Ying-Wu;Wang, Huamin;Niu, Wenjing;Lin, Ying-Wu;Xu, Jia-Kun
关键词：
-
Exploiting and Engineering Neuroglobin for Catalyzing Carbene N-H Insertions and the Formation of Quinoxalinones
作者：Sun, Li-Juan;Gao, Shu-Qin;Wen, Ge-Bo;Lin, Ying-Wu;Wang, Huamin;Lin, Ying-Wu;Xu, Jia-Kun
关键词：
-
Application of engineered heme enzymes based on myoglobin with high peroxidase activity for efficient degradation of various emerging pollutants
作者：Wu, Guang-Rong;Li, Ying-Fu;Lin, Ying-Wu;Xu, Jia-Kun;Sun, Li-Juan;Gao, Shu-Qin;Lin, Ying-Wu
关键词：Emerging pollutants; Heme enzyme; Myoglobin; Biodegradation
-
Phenoxazinone Synthase-like Activity of Rationally Designed Heme Enzymes Based on Myoglobin
作者：Sun, Li-Juan;Lang, Jia-Jia;Wen, Ge-Bo;Yuan, Hong;Tan, Xiangshi;Yuan, Hong;Tan, Xiangshi;Xu, Jia-Kun;Lin, Ying-Wu;Lin, Ying-Wu
关键词：
-
Surface Functionalization of SBA-15 for Immobilization of Myoglobin
作者：Miao, Hengmin;Li, Maosheng;Wang, Fang;Xu, Jiakun;Miao, Hengmin;Li, Jiao;Li, Maosheng;Lin, Ying-Wu;Lin, Ying-Wu
关键词：myoglobin; SBA-15; organic functionalization; protein immobilization; biocatalysis
-
Engineering globins for efficient biodegradation of malachite green: two case studies of myoglobin and neuroglobin
作者：Liu, Jiao;Wang, Xiao-Juan;Lin, Ying-Wu;Xu, Jia-Kun;Yuan, Hong;Tan, Xiang-Shi;Wang, Xiao-Juan;Gao, Shu-Qin;Wen, Ge-Bo;Lin, Ying-Wu
关键词：
