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Integrated mRNA and microRNA Transcriptome Sequencing Characterizes Sequence Variants and mRNA-microRNA Regulatory Networks in Grass Carp Fibroblasts Infected with Virulent and Attenuated GCRV

文献类型： 外文期刊

作者： Yao, Qiucheng 1 ; Zhang, Mengdi 1 ; Zu, Shaopo 2 ; Yang, Hong 3 ; Xie, Weitian 1 ; Chen, Jinjun 1 ; Chen, Zhibao 1 ; Ge, 1 ;

作者机构： 1.Guangdong Ocean Univ, Coll Coastal Agr Sci, Zhanjiang, Guangdong, Peoples R China

2.Haikou Customs Dist PR China, Haikou, Hainan, Peoples R China

3.Nanchang Anim Dis Prevent & Control Ctr, Nanchang, Jiangxi, Peoples R China

4.Foshan Univ, Sch Life Sci & Engn, Guangdong Prov Key Lab Anim Mol Design & Precise, Foshan 528231, Peoples R China

5.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Fishery Drug Dev, Minist Agr,Key Lab Aquat Anim Immune Technol, Guangzhou 510380, Peoples R China

关键词： Grass carp; GCRV; MiRNA; MRNA; Fibroblasts

期刊名称：MARINE BIOTECHNOLOGY （ 影响因子：2.798； 五年影响因子：3.0 ）

ISSN： 1436-2228

年卷期： 2021 年 23 卷 2 期

页码：

收录情况： SCI

摘要： Grass carp hemorrhagic disease is a fatal disease caused by the grass carp reovirus (GCRV). The aberrant regulation of transcripts has been implicated in many types of diseases. In the present study, we characterized mRNA and miRNA transcriptomes of different virulent GCRVs using RNA sequencing (RNA-Seq). One hundred eighteen miRNAs were identified as being differentially expressed between different virulent viruses in grass carp fibroblasts. Eight miRNAs were selected to verify the RNA-Seq results using RT-PCR and mRNA methods. In total, 996 differentially expressed mRNA genes were identified in grass carp fibroblasts, while 901 miRNA-mRNA target pairs were observed to be inversely regulated in grass carp fibroblasts. Integrated mRNA/miRNA expression profiling analysis results showed that the most influenced processes were the immune response and cell death. Three miRNAs were shown to exhibit the same expression patterns when two different methods were used and had important functions during viral infection. These results provide insights into the miRNA-mediated regulation of mRNA and valuable resources on transcript variation and regulation during GCRV infection, which are potentially useful for mechanistic and drug studies.

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