Expression characteristics of pineal miRNAs at ovine different reproductive stages and the identification of miRNAs targeting the AANAT gene
文献类型： 外文期刊
作者： Di, Ran 1 ; Liu, Qiu-Yue 1 ; Song, Shu-Hui 2 ; Tian, Dong-Mei 2 ; He, Jian-Ning 1 ; Ge, Ying 1 ; Wang, Xiang-Yu 1 ; Hu, W 1 ;
作者机构： 1.Chinese Acad Agr Sci, Inst Anim Sci, Key Lab Anim Genet & Breeding & Reprod, Minist Agr & Rural Affairs, 2 Yuanmingyuan West Rd, Beijing 100193, Peoples R China
2.Natl Genom Data Ctr, Beijing, Peoples R China
3.Chinese Acad Sci, CAS Key Lab Genome Sci & Informat, Beijing Inst Genom, Beijing, Peoples R China
4.China Natl Ctr Bioinformat, Beijing, Peoples R China
5.Int Ctr Agr Res Dry Areas ICARDA, Small Ruminant Genom, Addis Ababa, Ethiopia
6.NingXia Acad Agr & Forestry Sci, Res Ctr Grass & Livestock, 590 East Yellow River Rd, Yinchuan 750002, Ningxia, Peoples R China
关键词： Sheep; Pineal gland; miRNAs; Anestrus; Breeding season
期刊名称：BMC GENOMICS （ 影响因子：3.594； 五年影响因子：4.093 ）
ISSN： 1471-2164
年卷期： 2021 年 22 卷 1 期
页码：
收录情况： SCI
摘要： BackgroundMany recent studies have shown that miRNAs play important roles in the regulation of animal reproduction, including seasonal reproduction. The pineal gland is a crucial hub in the regulation of seasonal reproduction. However, little is known about the expression characteristics of pineal miRNAs in different reproductive seasons (anestrus and breeding season). Therefore, the expression profiles and regulatory roles of ovine pineal miRNAs were investigated during different reproductive stages using Solexa sequencing technology and dual luciferase reporter assays.ResultsA total of 427 miRNAs were identified in the sheep pineal gland. Significant differences in miRNA expression were demonstrated between anestrus and the breeding season in terms of the frequency distributions of miRNA lengths, number of expressed miRNAs, and specifically and highly expressed miRNAs in each reproductive stage. KEGG analysis of the differentially expressed (DE) miRNAs between anestrus and the breeding season indicated that they are significantly enriched in pathways related to protein synthesis, secretion and uptake. Furthermore, transcriptome analysis revealed that many target genes of DE miRNAs in the ribosome pathway showed relatively low expression in the breeding season. On the other hand, analyses combining miRNA-gene expression data with target relationship validation in vitro implied that miR-89 may participate in the negative regulation of aralkylamine N-acetyltransferase (AANAT) mRNA expression by targeting its 3'UTR at a unique binding site.ConclusionsOur results provide new insights into the expression characteristics of sheep pineal miRNAs at different reproductive stages and into the negative regulatory effects of pineal miRNAs on AANAT mRNA expression.
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