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vB_EfaS-DELF1, a novel Siphoviridae bacteriophage with highly effective lytic activity against vancomycin-resistant Enterococcus faecalis

文献类型： 外文期刊

作者： Soleimani-Delfan, Abbas 1 ; Bouzari, Majid 1 ; Wang, Ran 2 ;

作者机构： 1.Univ Isfahan, Fac Biol Sci & Technol, Dept Cell & Mol Biol & Microbiol, Hezar Jereeb St, Esfahan 8174673441, Iran

2.Jiangsu Acad Agr Sci, Inst Food Safety & Nutr, State Key Lab Cultivat Base MOST, Nanjing, Peoples R China

关键词： Enterococcus faecalis; Vancomycin-resistant; Siphoviridae; Phage therapy

期刊名称：VIRUS RESEARCH （ 影响因子：2.934； 五年影响因子：2.775 ）

ISSN： 0168-1702

年卷期： 2021 年 298 卷

页码：

收录情况： SCI

摘要： Enterococcus faecalis is an environmental agent of bovine mastitis in cows and has many cytopathic effects on the urinary tract in both humans and animals. In this study, a novel lytic bacteriophage, vB_EfaS-DELF1, was isolated against 21 E. faecalis isolated from bovine mastitis, including vancomycin-resistant E. faecalis (VRE). vB_EfaSDELF1 bacteriophage was specific for E. faecalis and showed no lytic effects against other tested Enterococcus spp., Gram-negative, or Gram-positive bacteria. Moreover, no activity was observed against yogurt starters. The phage suspension was stable in a wide range of pH, salinity, and temperature. It retained its activity in 3.5 % fat milk. vB_EfaS-DELF1 has the common phenotypic features of Siphoviridae with a double-strand DNA of 40,248 bp in length and a G + C content of 34.9 %. The genome encodes 62 putative ORFs and no tRNA. No undesirable genes such as lysogenic mediators, antibiotic resistance, or virulence factor genes were detected in the genome. The comparative genomic analysis demonstrated similarity to the other available phage genomes. The highest similarity was observed with two other phages (50 % coverage and 82.38 % identities with IME-EFm1; 35 % coverage and 86.22 % identities with IME-EFm5) that were placed in the same clade. The differences with the other aligned phages were high and were placed in distant clusters. Regarding the specificity of this new bacteriophage against all of the tested E. faecalis isolates and, in particular, against the vancomycin-resistant ones, and also the absence of antibiotic resistance or virulence genes in its genome, vB_EfaS-DELF1 is suggested as a potential candidate for biocontrol of E. faecalis infections.

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