Identification and characterization of alkaline phosphatase gene phoX in Microcystis aeruginosa PCC7806
文献类型： 外文期刊
作者： Hong, Sujuan 1 ; Pan, Qianhui 1 ; Chen, Siyu 1 ; Zu, Yao 1 ; Xu, Chongxin 2 ; Li, Jianhong 1 ;
作者机构： 1.Nanjing Normal Univ, Sch Life Sci, Nanjing 210023, Peoples R China
2.Jiangsu Acad Agr Sci, Inst Food Safety & Nutr, Minist Sci & Technol, Lab Food Qual,Safety State Key Lab Cultivat Bas, Nanjing 210014, Peoples R China
关键词： PhoX; Alkaline phosphatase; Microcystis; Phosphorus metabolism; Cyanobacterial bloom
期刊名称：3 BIOTECH （ 影响因子：1.798； 五年影响因子：2.389 ）
ISSN： 2190-572X
年卷期： 2021 年 11 卷 5 期
页码：
收录情况： SCI
摘要： PhoX is an extracellular alkaline phosphatase that is widely found in cyanobacteria and plays an important role in the conversion of extracellular organophosphorus into soluble inorganic phosphorus. However, the phoX gene has not yet been experimentally confirmed to exist in bloom-forming Microcystis species. In this study, we identified a putative phoX gene (GenBank accession no. ARI79942.1) in M. aeruginosa PCC7806 and overexpressed it in Escherichia coli 21 (DE3). The expressed PhoX protein displayed phosphodiesterase and phosphomonoesterase activities. In contrast to other bacterial PhoX proteins, which are activated mainly by Ca2+, Microcysits PhoX was most strongly activated by Mg2+, followed by Co2+, Ca2+, Zn2+ and Mn2+, but it was inhibited by Ni2+. Sequence analysis showed that phoX was highly conserved in the Microcystis genus (DNA similarity > 96% between species). phoX expression responded significantly to different environmental phosphorus levels. When PCC7806 cells were cultured in phosphorus-deficient medium (BG11-P), phoX expression reached its highest level at 2 h and then decreased to a low level at 4 h. Organophosphate induced the expression of phoX; its expression reached the highest level at 4 h and was maintained at a high level at 6 h. Our results confirmed a putative phoX gene and demonstrated that the phoX gene of Microcystis is conserved.
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