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Molecular identification and functional analysis of MyD88 in giant freshwater prawn (Macrobrachium rosenbergii) and expression changes in response to bacterial challenge

文献类型： 外文期刊

作者： Gao, Quanxin 1 ; Tang, Qiongying 1 ; Xia, Zhenglong 5 ; Yi, Shaokui 1 ; Cai, Miuying 5 ; Du, Houkuan 5 ; Yang, Jie 5 ; Li 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Zhejiang Prov Key Lab Aquat Resources Conservat &, Beijing, Peoples R China

2.Chinese Acad Fishery Sci, Key Lab Aquat Anim Genet Breeding & Nutr, Beijing, Peoples R China

3.Huzhou Univ, Huzhou Cent Hosp, Huzhou 313000, Peoples R China

4.Huzhou Univ, Coll Life Sci, Huzhou 313000, Peoples R China

5.Jiangsu Shufeng Prawn Breeding Co LTD, Gaoyou 225654, Peoples R China

关键词： MyD88; Macrobrachium rosenbergii; Cloning

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：5.162； 五年影响因子：5.137 ）

ISSN： 0141-8130

年卷期： 2021 年 178 卷

页码：

收录情况： SCI

摘要： Myeloid differentiation factor 88 (MyD88) is a crucial adaptor protein for Toll-like receptor (TLR)-mediated signaling pathways and plays an important role in immune response. In this study, the full-length cDNA of MyD88 from Macrobrachium rosenbergii (MRMyD88) was cloned. The MRMyD88 cDNA is 1758 bp long and contains a 1398-bp open reading frame. Multiple sequence alignment and phylogenetic analysis revealed that the amino acid sequence of MRMyD88 shared high identity with the known MyD88 proteins. The MRMyD88 mRNA was widely expressed in all examined tissues, with highest level in intestine, followed by gonad and pleopod. Furthermore, the MRMyD88 promoter region, spanning 1622 bp, contains several transcription factor-binding sites, including nine GATA-1 box motifs. Electrophoretic mobility shift assay showed that Gfi-1, SRF, and Oct-1 bind to the upstream region of MRMyD88. Additionally, the results showed that the expression levels of TLR1, TLR2 and TLR3 were different in response to Vibrio anguillarum, Lactobacillus plantarum and Aeromonas hydrophila infections. However, these bacteria significantly increased the expression levels of MyD88 and prophenoloxidase. These data suggest that the TLR-mediated signaling pathway is MyD88-dependent in response to pathogenic and probiotic bacteria in M. rosenbergii. (c) 2021 Published by Elsevier B.V.

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