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Transcriptome-wide N6-methyladenosine modification profiling of long non-coding RNAs during replication of Marek's disease virus in vitro

文献类型： 外文期刊

作者： Sun, Aijun 1 ; Zhu, Xiaojing 1 ; Liu, Ying 1 ; Wang, Rui 1 ; Yang, Shuaikang 1 ; Teng, Man 2 ; Zheng, Luping 2 ; Luo, Jun 2 ;

作者机构： 1.Henan Agr Univ, Coll Vet Med, Zhengzhou 450002, Henan, Peoples R China

2.Henan Acad Agr Sci, Minist Agr & Rural Affairs, Key Lab Anim Immunol, Zhengzhou 450002, Peoples R China

3.Henan Acad Agr Sci, Henan Prov Key Lab Anim Immunol, Zhengzhou 450002, Peoples R China

4.Henan Acad Agr Sci, UK China Ctr Excellence Res Avian Dis, Zhengzhou 450002, Peoples R China

5.Henan Univ Sci & Technol, Coll Anim Sci & Technol, Luoyang 471003, Peoples R China

关键词： Marek’ s disease virus; Long non-coding RNA; m(6)A; MeRIP-Seq; KEGG

期刊名称：BMC GENOMICS （ 影响因子：3.594； 五年影响因子：4.093 ）

ISSN： 1471-2164

年卷期： 2021 年 22 卷 1 期

页码：

收录情况： SCI

摘要： Background The newly discovered reversible N6-methyladenosine (m(6)A) modification plays an important regulatory role in gene expression. Long non-coding RNAs (lncRNAs) participate in Marek's disease virus (MDV) replication but how m(6)A modifications in lncRNAs are affected during MDV infection is currently unknown. Herein, we profiled the transcriptome-wide m(6)A modification in lncRNAs in MDV-infected chicken embryo fibroblast (CEF) cells. Results Methylated RNA immunoprecipitation sequencing results revealed that the lncRNA m(6)A modification is highly conserved with MDV infection increasing the expression of lncRNA m(6)A modified sites compared to uninfected cell controls. Gene Ontology and the Kyoto Encyclopedia of Genes and Genomes pathway analysis revealed that lncRNA m(6)A modifications were highly associated with signaling pathways associated with MDV infection. Conclusions In this study, the alterations seen in transcriptome-wide m(6)A occurring in lncRNAs following MDV-infection suggest this process plays important regulatory roles during MDV replication. We report for the first time profiling of the alterations in transcriptome-wide m(6)A modification in lncRNAs of MDV-infected CEF cells.

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