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Mitophagy is involved in the mitochondrial dysfunction of vitrified porcine oocytes

文献类型： 外文期刊

作者： Xu, Jiehuan 1 ; Zhang, Defu 2 ; Ju, Shiqiang 1 ; Sun, Lingwei 2 ; Zhang, Shushan 2 ; Wu, Caifeng 2 ; Rui, Rong 1 ; Dai, J 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Vet Med, Nanjing 210095, Jiangsu, Peoples R China

2.Shanghai Acad Agr Sci, Inst Anim Husb & Vet Sci, Shanghai 201106, Peoples R China

3.Shanghai Municipal Key Lab Agrigenet & Breeding, Div Anim Genet Engn, Shanghai, Peoples R China

4.Shanghai Engn Res Ctr Breeding Pig, Shanghai, Peoples R China

关键词： chloroquine; mitochondrial function; mitophagy; porcine oocyte; vitrification

期刊名称：MOLECULAR REPRODUCTION AND DEVELOPMENT （ 影响因子：2.823； 五年影响因子：3.13 ）

ISSN： 1040-452X

年卷期：

页码：

收录情况： SCI

摘要： Mitochondrial dysfunction is considered a crucial factor aggravating oocyte viability after vitrification-warming. To clarify the role of mitophagy in mitochondrial extinction of vitrified porcine oocytes, mitochondrial function, ultrastructural characteristics, mitochondria-lysosomes colocalization, and mitophagic proteins were detected with or without chloroquine (CQ) treatment. The results showed that vitrification caused mitochondrial dysfunction, including increasing reactive oxygen species production, decreasing mitochondrial membrane potential, and mitochondrial DNA copy number. Damaged mitochondrial cristae and mitophagosomes were observed in vitrified oocytes. A highly fused fluorescence distribution of mitochondria and lysosomes was also observed. In the detection of mitophagic flux, mitophagy was demonstrated as increasing fluorescence aggregation of microtubule-associated protein light chain 3B (LC3B), enhanced colocalization between LC3B, and voltage-dependent anion channels 1 (VDAC1), and upregulated LC3B-II/I protein expression ratio. CQ inhibited the degradation of mitophagosomes in vitrified oocytes, manifested as decreased mitochondria-lysosomes colocalization, increased fluorescence fraction of VDAC1 overlapping LC3B, increased LC3B-II/I protein expression ratio, and p62 accumulation. The inhibition of mitophagosomes degradation by CQ aggravated mitochondrial dysfunction, including increased oxidative damage, reduced mitochondrial function, and further led to loss of oocyte viability and developmental potentiality. In conclusion, mitophagy is involved in the regulation of mitochondrial function during porcine oocyte vitrification.

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