文献类型： 外文期刊
作者： Xu, Jiehuan 1 ; Zhang, Defu 2 ; Ju, Shiqiang 1 ; Sun, Lingwei 2 ; Zhang, Shushan 2 ; Wu, Caifeng 2 ; Rui, Rong 1 ; Dai, J 1 ;
作者机构： 1.Nanjing Agr Univ, Coll Vet Med, Nanjing 210095, Jiangsu, Peoples R China
2.Shanghai Acad Agr Sci, Inst Anim Husb & Vet Sci, Shanghai 201106, Peoples R China
3.Shanghai Municipal Key Lab Agrigenet & Breeding, Div Anim Genet Engn, Shanghai, Peoples R China
4.Shanghai Engn Res Ctr Breeding Pig, Shanghai, Peoples R China
关键词： chloroquine; mitochondrial function; mitophagy; porcine oocyte; vitrification
期刊名称：MOLECULAR REPRODUCTION AND DEVELOPMENT （ 影响因子：2.823； 五年影响因子：3.13 ）
ISSN： 1040-452X
年卷期：
页码：
收录情况： SCI
摘要： Mitochondrial dysfunction is considered a crucial factor aggravating oocyte viability after vitrification-warming. To clarify the role of mitophagy in mitochondrial extinction of vitrified porcine oocytes, mitochondrial function, ultrastructural characteristics, mitochondria-lysosomes colocalization, and mitophagic proteins were detected with or without chloroquine (CQ) treatment. The results showed that vitrification caused mitochondrial dysfunction, including increasing reactive oxygen species production, decreasing mitochondrial membrane potential, and mitochondrial DNA copy number. Damaged mitochondrial cristae and mitophagosomes were observed in vitrified oocytes. A highly fused fluorescence distribution of mitochondria and lysosomes was also observed. In the detection of mitophagic flux, mitophagy was demonstrated as increasing fluorescence aggregation of microtubule-associated protein light chain 3B (LC3B), enhanced colocalization between LC3B, and voltage-dependent anion channels 1 (VDAC1), and upregulated LC3B-II/I protein expression ratio. CQ inhibited the degradation of mitophagosomes in vitrified oocytes, manifested as decreased mitochondria-lysosomes colocalization, increased fluorescence fraction of VDAC1 overlapping LC3B, increased LC3B-II/I protein expression ratio, and p62 accumulation. The inhibition of mitophagosomes degradation by CQ aggravated mitochondrial dysfunction, including increased oxidative damage, reduced mitochondrial function, and further led to loss of oocyte viability and developmental potentiality. In conclusion, mitophagy is involved in the regulation of mitochondrial function during porcine oocyte vitrification.
- 相关文献
作者其他论文 更多>>
-
Detection and Analysis of Antidiarrheal Genes and Immune Factors in Various Shanghai Pig Breeds
作者：Zhou, Jinyong;Liu, Fuqin;He, Mengqian;Gao, Jun;Wu, Caifeng;Dai, Jianjun;Sun, Lingwei;Zhou, Jinyong;Liu, Fuqin;He, Mengqian;Gao, Jun;Wu, Caifeng;Sun, Lingwei;Gan, Yeqing;Bian, Yi;Wei, Jinliang;Zhang, Weijian;Zhang, Wengang;Han, Xuejun;Dai, Jianjun
关键词：Shanghai white pig; Fengjing pig; Shawutou pig; Meishan pig; Pudong white pig; gene; immunity factors
-
Characterization and Comparative Analysis of Whole-Transcriptome Sequencing in High- and Low-Fecundity Chongming White Goat Ovaries during the Estrus Phase
作者：Lin, Yuexia;Sun, Lingwei;Dai, Jianjun;Lv, Yuhua;Liao, Rongrong;Shen, Xiaohui;Gao, Jun;Sun, Lingwei;Dai, Jianjun;Gao, Jun;Sun, Lingwei;Dai, Jianjun;Gao, Jun
关键词：goat; ovary; fecundity; non-coding RNAs; ceRNA
-
PAK1-Dependent Regulation of Microtubule Organization and Spindle Migration Is Essential for the Metaphase I-Metaphase II Transition in Porcine Oocytes
作者：Peng, Lei;He, Yijing;Wang, Weihan;Li, Qiao;Ju, Shiqiang;Dai, Jianjun
关键词：porcine oocytes; P21-activated kinase 1; metaphase I-metaphase II transition; spindle dynamics
-
Transcriptomic and metabolomic dissection of skeletal muscle of crossbred Chongming white goats with different meat production performance
作者：Lin, Yuexia;Sun, Lingwei;Lv, Yuhua;Liao, Rongrong;Zhang, Shushan;Xu, Jiehuan;He, Mengqian;Wu, Caifeng;Zhang, Defu;Dai, Jianjun;Gao, Jun;Lin, Yuexia;Sun, Lingwei;Lv, Yuhua;Liao, Rongrong;Zhang, Keqing;Zhou, Jinyong;Zhang, Shushan;Xu, Jiehuan;He, Mengqian;Wu, Caifeng;Zhang, Defu;Dai, Jianjun;Gao, Jun;Sun, Lingwei;Zhang, Shushan;Xu, Jiehuan;He, Mengqian;Wu, Caifeng;Zhang, Defu;Dai, Jianjun;Gao, Jun;Shen, Xiaohui
关键词：RNA-seq; Differentially expressed gene; Liquid chromatography-mass spectrometry; Goat meat development
-
Dietary Supplementation with Rumen-Protected Arginine or N-Carbamylglutamate Enhances Fetal Liver Development in Nutrient-Restricted Pregnant Hu Ewes
作者：Lin, Yuexia;Sun, Lingwei;He, Mengqian;Xu, Jiehuan;Wu, Caifeng;Gao, Jun;Dai, Jianjun;Sun, Lingwei;He, Mengqian;Xu, Jiehuan;Wu, Caifeng;Gao, Jun;Dai, Jianjun
关键词：maternal undernutrition; rumen-protected arginine (RP-Arg); N-carbamylglutamate (NCG); fetal liver function; antioxidant; apoptosis
-
Characterization of Meat Metabolites and Lipids in Shanghai Local Pig Breeds Revealed by LC-MS-Based Method
作者：Gao, Jun;Sun, Lingwei;Tu, Weilong;Zhang, Shushan;Xu, Jiehuan;He, Mengqian;Zhang, Defu;Dai, Jianjun;Wu, Caifeng;Gao, Jun;Sun, Lingwei;Tu, Weilong;Zhang, Shushan;Zhang, Defu;Dai, Jianjun;Wu, Caifeng;Gao, Jun;Sun, Lingwei;Cao, Mengqian;Zhang, Shushan;Xu, Jiehuan;He, Mengqian;Zhang, Defu;Dai, Jianjun;Wu, Caifeng;Gao, Jun;Sun, Lingwei;Tu, Weilong;Zhang, Shushan;Xu, Jiehuan;He, Mengqian;Zhang, Defu;Dai, Jianjun;Cao, Mengqian;Wu, Xiao
关键词：metabolomics; lipidomics; liquid chromatography-mass spectrometry; longissimus dorsi muscle; gluteus muscle
-
Evaluation of Dry Ice for Short-Term Storage and Transportation of Frozen Boar Semen
作者：He, Mengqian;Sun, Lingwei;Xu, Jiehuan;Wu, Caifeng;Zhang, Shushan;Gao, Jun;Zhang, Defu;Dai, Jianjun;He, Mengqian;Sun, Lingwei;Xu, Jiehuan;Wu, Caifeng;Zhang, Shushan;Gao, Jun;Zhang, Defu;Dai, Jianjun;He, Mengqian;Sun, Lingwei;Xu, Jiehuan;Wu, Caifeng;Zhang, Shushan;Zhang, Defu;Han, Xuejun;Dai, Jianjun;Gan, Yeqing;Bian, Yi;Wei, Jinliang;Zhang, Weijian;Zhang, Wengang
关键词：semen cryopreservation; dry ice; semen transportation; boar
