文献类型： 外文期刊
作者： Xu, Xinyan 1 ; Liu, Liu 1 ; Xu, Lihui 2 ; Zhang, Yang 1 ; Hafeez, Rahila 1 ; Ijaz, Munazza 1 ; Ali, Hayssam M. 3 ; Shahid, Muhammad Shafiq 4 ; Ahmed, Temoor 1 ; Ondrasek, Gabrijel 7 ; Li, Bin 1 ;
作者机构： 1.Zhejiang Univ, Inst Biotechnol, State Key Lab Rice Biol & Breeding,Zhejiang Key La, Minist Agr,Key Lab Mol Biol Crop Pathogens & Insec, Hangzhou 310058, Peoples R China
2.Shanghai Acad Agr Sci, Inst Ecoenvironm Protect, Shanghai 201403, Peoples R China
3.King Saud Univ, Coll Sci, Dept Bot & Microbiol, Riyadh 11451, Saudi Arabia
4.Sultan Qaboos Univ, Coll Agr & Marine Sci, Dept Plant Sci, Al Khoud 123, Muscat, Oman
5.Xianghu Lab, Hangzhou 311231, Peoples R China
6.Western Caspian Univ, Dept Life Sci, Baku, Azerbaijan
7.Univ Zagreb, Fac Agr, Svetosimunska Cesta 25, Zagreb 10000, Croatia
关键词： Dct system; Burkholderia seminalis; Anti-fungus; C4-dicarboxylates; Cyclo (Phe-Pro)
期刊名称：MICROBIAL CELL FACTORIES （ 影响因子：4.9； 五年影响因子：6.1 ）
ISSN：
年卷期： 2024 年 23 卷 1 期
页码：
收录情况： SCI
摘要： Cyclo (Phe-Pro) (cFP), a cyclic dipeptide with notable antifungal, antibacterial, and antiviral properties, shows great promise for biological control of plant diseases. Produced as a byproduct by non-ribosomal peptide synthetases (NRPS), the regulatory mechanism of cFP biosynthesis remains unclear. In a screening test of 997 Tn5 mutants of Burkholderia seminalis strain R456, we identified eight mutants with enhanced antagonistic effects against Fusarium graminearum (Fg). Among these, mutant 88's culture filtrate contained cFP, confirmed through HPLC and LC-MS, which actively inhibited Fg. The gene disrupted in mutant 88 is part of the Dct transport system (Dct-A, -B, -D), responsible for C4-dicarboxylate transport. Knockout mutants of Dct genes exhibited higher cFP levels than the wild type, whereas complementary strains showed no significant difference. Additionally, the presence of exogenous C4-dicarboxylates reduced cFP production in wild type R456, indicating that these substrates negatively regulate cFP synthesis. Given that cFP synthesis is related to NRPS, we previously identified an NRPS cluster in R456, horizontally transferred from algae. Specifically, knocking out gene 2061 within this NRPS cluster significantly reduced cFP production. A Fur box binding site was predicted upstream of gene 2061, and yeast one-hybrid assays confirmed Fur protein binding, which increased with additional C4-dicarboxylates. Knockout of the Fur gene led to up-regulation of gene 2061 and increased cFP production, suggesting that C4-dicarboxylates suppress cFP synthesis by enhancing Fur-mediated repression of gene 2061.
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