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Equus beta-Defensin-1 Regulates Innate IMMUNE Response in S. aureus-Infected Mouse Monocyte Macrophage

文献类型： 外文期刊

作者： Pei, Le 1 ; Liu, Kun 3 ; Wei, Wei 1 ; Su, Hong 1 ; Li, Feng 2 ; Feng, Ying 2 ; Wang, Daqing 2 ; Li, Xiunan 2 ; Hou, Yongyue 2 ; Cao, Guifang 1 ;

作者机构： 1.Inner Mongolia Agr Univ, Coll Vet, Inner Mongolia Key Lab Basic Vet Med, Hohhot 010010, Peoples R China

2.Inner Mongolia Acad Agr & Anim Husb Sci, Hohhot 010010, Peoples R China

3.Inner Mongolia Med Univ, Sch Publ Hlth, Hohhot 010010, Peoples R China

关键词： beta-defensin-1; Equus; S. aureus-infected; immune response

期刊名称：ANIMALS （ 影响因子：3.231； 五年影响因子：3.312 ）

ISSN： 2076-2615

年卷期： 2022 年 12 卷 21 期

页码：

收录情况： SCI

摘要： Beta-defensin-1 (BD-1) is among the class of antibacterial peptides that are rich in disulfide bonds, have direct antibacterial activity and showed enhanced expression following external stimulation. However, existing research studies only treated BD-1 to cell models without stimulation from pathogen-associated molecular patterns (PAMPs), which will further influence our understanding of the role of BD-1. In this study, we map the tissue distribution of Equus BD-1 (i.e., eBD-1, ass ED-1, and mule BD-1) and compare their expression levels in various tissues. We further characterize the three kinds of Equus BD-1 by analyzing their full-length cDNA. We showed that eBD-1, ass ED-1, and mule ED-1 have an identical (100%) open reading frame (ORF). The ORF encoding OEBD-1 expressed the ORF in the E. coli Top10 expression system. This expression system was combined with an S. aureus-infected J774A.1 macrophage cell line to determine the influence on innate immune mediator expression. Using this expression model system, it was determined that the OEBD-1 protein enhanced IL-6 and TNF-alpha secretion. It can also promote TLR2, CCL2, CCL7, CXCL10 and NF-kappa B p65 mRNA expression. Moreover, OEBD-1 upregulates phosphorylation of ATK, Syk and I kappa B-alpha. In addition, OEBD-1 enhances the macrophage's ability to phagocytose S. aureus. In conclusion, Equus BD-1 was shown to play an essential role in macrophage-involved innate immune responses in an in vitro system.

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