Detection and determination of spectinomycin and lincomycin in poultry muscles and pork by ASE-SPE-GC-MS/MS
文献类型： 外文期刊
作者： Guo, Yawen 1 ; Xie, Xing 3 ; Diao, Zhixiang 1 ; Wang, Yajuan 1 ; Wang, Bo 2 ; Xie, Kaizhou 1 ; Wang, Xutang 1 ; Zhang, Pe 1 ;
作者机构： 1.Yangzhou Univ, Coll Anim Sci & Technol, Yangzhou 225009, Jiangsu, Peoples R China
2.Yangzhou Univ, Joint Int Res Lab Agr & Agri Prod Safety, Yangzhou 225009, Jiangsu, Peoples R China
3.Jiangsu Acad Agr Sci, Inst Vet Med, Minist Agr, Key Lab Vet Biol Engn & Technol, Nanjing 210014, Peoples R China
4.Yangzhou Univ, Coll Vet Med, Yangzhou 225009, Jiangsu, Peoples R China
关键词： Spectinomycin and lincomycin; Poultry muscles; Pork; Accelerated solvent extraction; Gas chromatography-tandem mass spectrom-etry; Solid-phase extraction
期刊名称：JOURNAL OF FOOD COMPOSITION AND ANALYSIS （ 影响因子：4.556； 五年影响因子：4.89 ）
ISSN： 0889-1575
年卷期： 2021 年 101 卷
页码：
收录情况： SCI
摘要： An innovative study on the qualitative identification and quantitative determination of spectinomycin and lincomycin residues in poultry (chicken, duck, and goose) muscles and pork using gas chromatography-tandem mass spectrometry (GC-MS/MS) was conducted. The samples were extracted and separated with accelerated solvent extraction (ASE) and then purified and enriched by solid-phase extraction (SPE). Then, the obtained residues were derivatized with 200 mu L bis(trimethylsilyl) trifluoroacetamide (BSTFA) for 60 min at 75 degrees C, and the derivative products were injected into a GC-MS/MS system. Under the optimized conditions, the limits of detection (LODs) and limits of quantification (LOQs) of spectinomycin and lincomycin in samples were 2.5-4.6 mu g/kg and 5.7-7.6 mu g/kg, respectively, the targets' recoveries were 79.7 %-94.2 %, the determination coefficients were 0.9992-0.9998, and the relative standard deviations (RSDs) were 1.2 %-3.5 %. The method revealed good linearity, and the parameters met the European Union requirements for the detection of veterinary drug residues. Poultry muscles and pork were procured from local stores for actual sample testing, confirming the feasibility and practicability of this method. Therefore, this study established an ASE-SPE-GC-MS/MS method to detect spectinomycin and lincomycin residues in animal-derived food.
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