文献类型： 外文期刊

作者： Niu, Naiqi ¹ ; Zhao, Runze ¹ ; Tian, Ming ² ; Zong, Wencheng ¹ ; Hou, Xinhua ¹ ; Liu, Xin ¹ ; Wang, Ligang ¹ ; Wang, Lixian ¹ ; Zhang, Longchao ¹ ;

作者机构： 1.Chinese Acad Agr Sci CAAS, Inst Anim Sci, State Key Lab Anim Biotech Breeding, Beijing 100193, Peoples R China

2.Heilongjiang Acad Agr Sci, Inst Anim Husb, Harbin 150086, Peoples R China

关键词： genome-wide association study; CD4+/CD8+; CD4-CD8+CD3+; platelet count; SNP

期刊名称：ANIMALS （ 影响因子：2.7； 五年影响因子：3.2 ）

ISSN： 2076-2615

年卷期： 2024 年 14 卷 21 期

页码：

收录情况： SCI

摘要： A genome-wide association study (GWAS) was conducted on 489 F2 pigs froma cross between Large White boars and Min pig sows, focusing on haematological parameters and T lymphocyte subpopulations (17 traits). Using the Illumina PorcineSNP60 Genotyping BeadChip, significant SNP loci were identified, particularly onSus scrofachromosome 7 (SSC7) for platelet count(PLT) and plateletocrit (PCT), with glutamate metabotropic receptor 4 (GRM4) as a candidate gene forPLT. White blood cell count (WBC) showed a significant association with SSC12. For T lymphocytetraits, significant signals were found on SSC7, SSC2, and SSC5, with candidate genes including,chloride intracellular channel 5 (CLIC5), tripartite motif containing 15 (TRIM15), and solute carrierfamily 17 member 4 (SLC17A4). Gene ontology (GO) enrichment analysis highlighted the MHC classII protein complex binding pathway as the most significant for immune traits. These findings offervaluable insights for breeding disease-resistant pigs and understanding immune-related traits in bothpigs and potentially humans. The breeding of disease-resistant pigs has consistently been a topic of significant interest and concern within the pig farming industry. The study of pig blood indicators has the potential to confer economic benefits upon the pig farming industry, whilst simultaneously providing valuable insights that can inform the study of human diseases. In this study, an F2 resource population of 489 individuals was generated through the intercrossing of Large White boars and Min pig sows. A total of 17 haematological parameters and T lymphocyte subpopulations were measured, including white blood cell count (WBC), lymphocyte count (LYM), lymphocyte count percentage (LYM%), monocyte count (MID), monocyte count percentage (MID%), neutrophilic granulocyte count (GRN), percentage of neutrophils (GRN%), mean platelet volume (MPV), platelet distribution width (PDW), platelet count (PLT), CD4+/CD8+, CD4+CD8+CD3+, CD4+CD8-CD3+, CD4-CD8+CD3+, CD4-CD8-CD3+, and CD3+. The Illumina PorcineSNP60 Genotyping BeadChip was obtained for all of the F2 animals. Subsequently, a genome-wide association study (GWAS) was conducted using the TASSEL 5.0 software to identify associated variants and candidate genes for the 17 traits. Significant association signals were identified for PCT and PLT on SSC7, with 1 and 11 significant SNP loci, respectively. A single nucleotide polymorphism (SNP) on SSC12 was identified as a significant predictor of the white blood cell (WBC) trait. Significant association signals were detected for the T lymphocyte subpopulations, namely CD4+/CD8+, CD4+CD8+CD3+, CD4+CD8-CD3+, and CD4-CD8+CD3+, with the majority of these signals observed on SSC7. The genes CLIC5, TRIM15, and SLC17A4 were identified as potential candidates for influencing CD4+/CD8+ and CD4-CD8+CD3+. A missense variant, c.2707 G>A, in the SLC17A4 gene has been demonstrated to be significantly associated with the CD4+/CD8+ and CD4-CD8+CD3+ traits. Three missense variants (c.425 A>C, c.500 C>T, and c.733 A>G) have been identified in the TRIM15 gene as being linked to the CD4+/CD8+ trait. Nevertheless, only c.425 A>C has been demonstrated to be significantly associated with CD4-CD8+CD3+. In the CLIC5 gene, one missense variant (c.957 T>C) has been identified as being associated with the CD4+/CD8+ and CD4-CD8+CD3+ traits. Additionally, significant association signals were observed for CD4+CD8+CD3+ and CD4+CD8-CD3+ on SSC2 and 5, respectively. Subsequently, a gene ontology (GO) enrichment analysis was conducted on all genes within the quantitative trait loci (QTL) intervals of platelet count, CD4+/CD8+, and CD4-CD8+CD3+. The MHC class II protein complex binding pathway was identified as the most significant pathway among the three immune traits. These results provide guidance for further research in the field of breeding disease-resistant pigs.