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Study of the properties of carotenoids and key carotenoid biosynthesis genes from Deinococcus xibeiensis R13

文献类型： 外文期刊

作者： Chu, Xiaoting 1 ; Liu, Jie 2 ; Gu, Wanyi 2 ; Tian, Liqing 1 ; Tang, Susu 3 ; Zhang, Zhidong 4 ; Jiang, Ling 5 ; Xu, Xian 2 ;

作者机构： 1.Nanjing Tech Univ, Coll Biotechnol & Pharmaceut Engn, State Key Lab Mat Oriented Chem Engn, Nanjing, Jiangsu, Peoples R China

2.Nanjing Normal Univ, Sch Food Sci & Pharmaceut Engn, Nanjing 210046, Jiangsu, Peoples R China

3.Nanjing Tech Univ, Sch Pharmaceut Sci, Nanjing, Jiangsu, Peoples R China

4.Xinjiang Uyghur Autonomous Reg, Inst Microbiol Xinjiang, Xinjiang Acad Agr Sci, Urumqi, Peoples R China

5.Nanjing Tech Univ, Coll Food Sci & Light Ind, Nanjing 211816, Jiangsu, Peoples R China

关键词： carotenoids; Deinococcus; free radical scavenging; key enzymes; stability

期刊名称：BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY （ 影响因子：2.431； 五年影响因子：2.124 ）

ISSN： 0885-4513

年卷期：

页码：

收录情况： SCI

摘要： To investigate the properties of carotenoids from the extremophile Deinococcus xibeiensis R13, the factors affecting the stability of carotenoids extracted from D. xibeiensis R13, including temperature, illumination, pH, redox chemicals, metal ions, and food additives, were investigated. The results showed that low temperature, neutral pH, reducing agents, Mn2+, and food additives (xylose and glucose) can effectively improve the stability of Deinococcus carotenoids. The carotenoids of D. xibeiensis R13 exhibited strong antioxidant activity, with the scavenging rate of hydroxyl radicals reaching 71.64%, which was higher than the scavenging efficiency for 1,1-diphenyl-2-picrylhydrazyl free radicals and 2,2'-azino-bis (3-ethyl-benzothiazoline-6-sulfonic acid) free radicals (44.55 and 27.65%, respectively). In addition, the total antioxidant capacity reached 0.60 U/ml, which was 2.61-fold that of carotenoids from the model strain Deinococcus radiodurans R1. Finally, we predicted the gene clusters encoding carotenoid biosynthesis pathways in the genome of R13 and identified putative homologous genes. The key enzyme genes (crtE, crtB, crtI, crtLm, cruF, crtD, and crtO) in carotenoid synthesis of D. xibeiensis R13 were cloned to construct the multigene coexpression plasmids pET-EBI and pRSF-LmFDO. The carotenoid biosynthesis pathway was heterologously introduced into engineered Escherichia coli EBILmFDO, which exhibited a higher yield (7.14 mg/L) than the original strain. These analysis results can help us to better understand the metabolic synthesis of carotenoids in extremophiles.

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