Comparative transcriptome analysis provides insights into the resistance regulation mechanism and inhibitory effect of fungicide phenamacril in Fusarium asiaticum
文献类型： 外文期刊
作者： Zheng, Zhitian 1 ; Liu, Huaqi 1 ; Luo, Xiao 1 ; Liu, Runze 1 ; Joe, Alexander Dumbi 1 ; Li, Haolin 1 ; Sun, Haiyan 3 ; Lin, Yanling 4 ; Li, Yanzhong 2 ; Wang, Yunpeng 1 ;
作者机构： 1.Huaiyin Inst Technol, Sch Life Sci & Food Engn, Huaian 223003, Peoples R China
2.Lanzhou Univ, Coll Pastoral Agr Sci & Technol, State Key Lab Herbage Improvement & Grassland Agro, Lanzhou 730020, Peoples R China
3.Inst Plant Protect, Jiangsu Acad Agr Sci, Nanjng 210014, Peoples R China
4.Jiangsu GOOD HARVEST WEIEN Agrochem Co Ltd, Beijing 101318, Peoples R China
关键词： Comparative transcriptome; Phenamacril; Resistance regulation; Inhibitory effect; Fusarium asiaticum
期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：4.7； 五年影响因子：4.7 ）
ISSN： 0048-3575
年卷期： 2024 年 201 卷
页码：
收录情况： SCI
摘要： Fusarium asiaticum is a destructive phytopathogenic fungus that causes Fusarium head blight of wheat (FHB), leading to serious yield and economic losses to cereal crops worldwide. Our previous studies indicated that target -site mutations (K216R/E, S217P/L, or E420K/G/D) of Type I myosin FaMyo5 conferred high resistance to phenamacril. Here, we first constructed one sensitive strain H1S and three point mutation resistant strains HA, HC and H1R. Then we conducted comparative transcriptome analysis of these F. asiaticum strains after 1 and 10 mu g center dot mL-1 phenamacril treatment. Results indicated that 2135 genes were differentially expressed (DEGs) among the sensitive and resistant strains. The DEGs encoding ammonium transporter MEP1/MEP2, nitrate reductase, copper amine oxidase 1, 4-aminobutyrate aminotransferase, amino -acid permease inda1, succinatesemialdehyde dehydrogenase, 2, 3-dihydroxybenzoic acid decarboxylase, etc., were significantly up -regulated in all the phenamacril-resistant strains. Compared to the control group, a total of 1778 and 2097 DEGs were identified in these strains after 1 and 10 mu g center dot mL-1 phenamacril treatment, respectively. These DEGs involved in 4aminobutyrate aminotransferase, chitin synthase 1, multiprotein-bridging factor 1, transcriptional regulatory protein pro -1, amino -acid permease inda1, ATP -dependent RNA helicase DED1, acetyl -coenzyme A synthetase, sarcoplasmic/endoplasmic reticulum calcium ATPase 2, etc., showed significantly down -regulated expression in phenamacril-sensitive strain but not in resistant strains after phenamacril treatment. In addition, cyanide hydratase, mating -type protein MAT -1, putative purine nucleoside permease, plasma membrane protein yro2, etc., showed significantly co -down -regulated expression in all the strains after phenamacril treatment. Taken together, This study provides deep insights into the resistance regulation mechanism and the inhibitory effect of fungicide phenamacril and these new annotated proteins or enzymes are worth for the discovery of new fungicide targets.
- 相关文献
作者其他论文 更多>>
-
The critical roles of the Zn2 Cys6 transcription factor Fp487 in the development and virulence of Fusarium pseudograminearum : A potential target for Fusarium crown rot control
作者：Yang, Xiaoyue;Cao, Shulin;Sun, Haiyan;Deng, Yuanyu;Zhang, Xin;Chen, Huaigu;Li, Wei;Li, Yan;Ma, Dongfang;Cao, Shulin;Chen, Huaigu;Li, Wei
关键词：Zn 2 Cys 6 transcription factor; FCR; Wheat; Fusarium pseudograminearum; Virulence; Spray-induced gene silencing
-
Genome Analyses Reveal the Secondary Metabolites that Potentially Influence the Geographical Distribution of Fusarium pseudograminearum Populations
作者：Li, Wei;Cao, Shulin;Sun, Haiyan;Yang, Xiaoyue;Zhang, Xin;Deng, Yuanyu;Chen, Huaigu;Li, Wei;Chen, Huaigu;Xu, Lei;Pavlov, Igor N.;Litovka, Yulia A.;Pavlov, Igor N.;Litovka, Yulia A.
关键词：F. pseudograminearum; Fusarium crown rot; genome; mycotoxin; secondary metabolite biosynthesis gene cluster
-
A novel alternavirus with three dsRNA segments from Fusarium pseudograminearum, the pathogen of Fusarium crown rot in wheat
作者：Liu, Fuyu;Deng, Qingchao;Liu, Fuyu;Cao, Shulin;Sun, Haiyan;Chen, Huaigu;Li, Wei;Xia, Lele;Li, Wei
关键词：
-
Efficacy of cyclobutrifluram in controlling Fusarium crown rot of wheat and resistance risk of three Fusarium species to cyclobutrifluram
作者：Sun, Haiyan;Cai, Shiyan;Deng, Yuanyu;Cao, Shulin;Yang, Xiaoyue;Lu, Yanteng;Li, Wei;Chen, Huaigu;Sun, Haiyan;Deng, Yuanyu;Cao, Shulin;Li, Wei;Chen, Huaigu;Li, Wei;Chen, Huaigu
关键词：Fusarium crown rot; Cyclobutrifluram; TYMIRIUM (R) technology; Control efficacy; Resistance risk; Cross-resistance
-
FgSdhC Paralog Confers Natural Resistance toward SDHI Fungicides in Fusarium graminearum
作者：Sun, Haiyan;Cai, Shiyan;Li, Xinlei;Deng, Yuanyu;Yang, Xiaoyue;Cao, Shulin;Li, Wei;Chen, Huaigu;Sun, Haiyan;Chen, Huaigu;Liu, Huiquan
关键词：Fusarium graminearum; succinate dehydrogenasesubunitC; paralog; natural resistance; SDHI; molecular functions
-
Trehalase Inhibitor Validamycin May Have Additional Mechanisms of Toxicology against Rhizoctonia cerealis
作者：Yang, Xiaoyue;Shu, Yan;Cao, Shulin;Sun, Haiyan;Zhang, Xin;Zhang, Aixiang;Chen, Huaigu;Li, Wei;Yang, Xiaoyue;Li, Yan;Ma, Dongfang;Chen, Huaigu;Li, Wei
关键词：Rhizoctonia cerealis; sharp eyespot of wheat; validamycin; trehalase; ribosome
-
Spectral Quantitative Analysis and Research of Fusarium Head Blight Infection Degree in Wheat Canopy Visible Areas
作者：Chen, Yanyu;Wang, Xiaochan;Zhang, Xiaolei;Wang, Dezhi;Xu, Xin;Sun, Ye;Sun, Haiyan
关键词：in situ wheat canopy; fusarium head blight; spectrum; infection degree; visible areas
