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Effect of muscone on anti-apoptotic ability of muskrat prostate primary cells

文献类型： 外文期刊

作者： Zhang, Yu 1 ; Zhao, Xuefei 1 ; Man, Xin 1 ; Ma, Yue 1 ; Zhang, Wei 1 ; Wang, Yu 1 ; Lei, Xing 1 ; Bai, Suying 1 ;

作者机构： 1.Northeast Forestry Univ, Coll Wildlife & Protected Area, Harbin, Peoples R China

2.Chinese Acad Fishery Sci, Heilongjiang River Fisheries Res Inst, Natl & Local Joint Engn Lab Freshwater Fish Breedi, Harbin, Peoples R China

3.State Forestry & Grassland Adm, Detecting Ctr Wildlife, Harbin, Peoples R China

关键词： apoptosis; muscone; muskrat (Ondatra zibethicus); primary culture; prostate

期刊名称：AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY （ 影响因子：3.21； 五年影响因子：3.62 ）

ISSN： 0363-6119

年卷期： 2022 年 323 卷 4 期

页码：

收录情况： SCI

摘要： Muskrat is a small fur animal with a pair of scent glands that can secrete muskrat musk during breeding season. The consensus is muskrat musk functions as a pheromone, but we hypothesized it has a broader role. In previous research, we found the pres-ence of muscone in muskrat musk. To study whether the muscone can affect the apoptosis of muskrat prostate, we carried out the following investigations. Primary muskrat prostate cells were cultured and treated with muscone. Then we drew cell prolifera-tion curves by applying the CCK-8 and used TdT-mediated dUTP nick end labeling (TUNEL) to detect apoptosis. Levels of mRNA transcription and protein expression of Bcl-2 as well as Bax were detected by qRT-PCR and the Western blot. Meanwhile, we collected tissue samples of muskrat prostates and froze sections to analyze the fluorescence signal intensity of BCL-2 and BAX via immunofluorescence. Under the treatment of 30 mu mol/L muscone, the proliferation rate of the experimental group exceeded that of the control group, and the proportion of cells undergoing apoptosis was lower in the experimental group. The qRT-PCR and Western blot result showed that, in the experimental group, the ratio of Bcl-2 to Bax mRNA transcription levels increased by 2.85 times and their corresponding protein expression ratio increased by 2.37 times (P < 0.05). Immunofluorescence results were consistent with the cell experiment's results. The fluorescence signal intensity of BCL-2 was higher in the breeding season than nonbreeding season but vice versa for BAX. Based on these results, we speculate that the muscone could regulates pros-tate development by inhibiting apoptosis.

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