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Identification and characterization of a novel adenine phosphoribosyltransferase gene (ZmAPT2) from maize (Zea mays L.)

文献类型： 外文期刊

作者： Wu, Suowei 1 ; Yu, Zhanwang 1 ; Wang, Fengge 3 ; Li, Weihua 1 ; Yang, Qingkai 1 ; Ye, Chunjiang 1 ; Sun, Yan 1 ; Jin, Dem 1 ;

作者机构： 1.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Genom, Beijing 100101, Peoples R China

2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

3.Beijing Acad Agr & Forestry Sci,

关键词： maize;adenine phosphoribosyltransferase (APRT);RAGE;RT-PCR;ARABIDOPSIS-THALIANA;CYTOKININ METABOLISM;TOMATO PLANT;2ND FORM;ASSOCIATION;CLONING;APRT;TGMS;MUTANT;RICE

期刊名称：DNA SEQUENCE （ 影响因子：0.408； 五年影响因子：0.594 ）

ISSN：

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收录情况： SCI

摘要： Adenine phosphoribosyltransferase (APRT) is the key enzyme that converts adenine to adenosine monophosphate (AMP) in the purine salvage pathway. It was found that several different forms of APRT gene exist in plants, but no APRT gene in maize has been reported up to now. In this study, a novel maize APRT gene was cloned and characterized through a combination of bioinformatic, RT-PCR and RACE strategies. The full length of APRT cDNA sequence is 1202 nucleotides, with an ORF encoding 214 amino acid residues. Alignment of the deduced protein with that of other plant APRT genes indicates that the new gene is the form 2 of maize APRT, thus it was named ZmAPT2. Through basic local alignment search tool, search in the genomic survey sequence database of MaizeGDB, the putative genomic sequence of ZmAPT2 was obtained. Comparison of the cDNA and genomic sequence of the ZmAPT2 gene revealed that it contained seven exons and six introns. The locations of the introns within the maize ZmAPT2 coding region were consistent with those in the previously isolated APRTs of arabidopsis and rice. RT-PCR analysis showed that ZmAPRT was constitutively expressing in different organs under high temperature and salt stresses. Southern blot analysis indicated that at least three APRT genes existed in maize genome. These results confirmed that the novel maize ZmAPT2 gene was truly identified, and its potential role in maize growth and development was discussed.

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