您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Evaluation of reference genes for quantitative expression analysis in Mylabris sibirica (Coleoptera, Meloidae)

文献类型： 外文期刊

作者： Shen, Chen-Hui 1 ; Tang, Min 1 ; Li, Xiao-Fei 1 ; Zhu, Li 1 ; Li, Wei 2 ; Deng, Pan 3 ; Zhai, Qing 4 ; Wu, Gang 1 ; Yan, Xiao-Hong 1 ;

作者机构： 1.Chinese Acad Agr Sci, Oil Crops Res Inst, Key Lab Agr Genet Modified Organisms Traceabil,Min, Minist Agr & Rural Affairs,Supervis & Test Ctr Wuh, Wuhan 430062, Peoples R China

2.Chinese Acad Agr Sci, Ctr Sci & Technol Disseminat & Ind Dev, Northern Propagat Expt Stn, Oil Crops Res Inst, Wuhan, Peoples R China

3.Jiangsu Acad Agr Sci, Inst Leisure Agr, Nanjing, Peoples R China

4.Henan Agr Univ, Coll Plant Protect, Zhengzhou, Peoples R China

关键词： Mylabris sibirica; reference gene; RT-qPCR; RefFinder; geNorm

期刊名称：FRONTIERS IN PHYSIOLOGY （ 影响因子：4.0； 五年影响因子：4.7 ）

ISSN：

年卷期： 2024 年 15 卷

页码：

收录情况： SCI

摘要： Mylabris sibirica is a hypermetamorphic insect whose adults feed on oilseed rape. However, due to a shortage of effective and appropriate endogenous references, studies on molecular functional genes in Mylabris sibirica, have been tremendously limited. In this study, ten internal reference genes (ACT, ARF1, AK, EF1 alpha, GAPDH, alpha-TUB, RPL6, RPL13, RPS3 and RPS18) were tested and assessed under four selected treatments including adult ages, adult tissues, temperatures, and sex by RT-qPCR based on five methods (Ct value, geNorm, NormFinder, BestKeeper and RefFinder). Our findings showed that RPL6 and RPL13 were the most optimal internal reference gene combination for gene expression during various adult ages and under diverse temperatures; The combination of RPL6 and RPS18 was recommended to test gene transcription levels under different adult tissues. AK and RPL6 were the best reference genes in male and female adults. RPL6 and RPL13 were the most appropriate reference gene pair to estimate gene expression levels under four different tested backgrounds. The relative transcript levels of a uridine diphosphate (UDP)-N-acetylglucosamine-pyrophosphorylase (MsUAP), varied greatly according to normalization with the two most- and least-suited reference genes. This study will lay the basis for further molecular physiology and biochemistry studies in M. sibirica, such as development, reproduction, sex differentiation, cold and heat resistance.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台