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Cloning and Expression of the Porcine MEF2a Gene

文献类型： 外文期刊

作者： Zhang, Dong-jie 1 ; Gao, Zhi-sheng 2 ; Liu, Di 1 ; Yang, Guo-wei 1 ;

作者机构： 1.Heilongjiang Acad Agr Sci, Harbin 150086, Peoples R China

2.Harbin Normal Univ, Coll Life Sci & Biol Engn, Harbin 150080, Peoples R China

关键词： Methods and Techniques;Molecular Genetics: Biochemistry and Molecular Biophysics;Population Genetics: Population Studies

期刊名称：JOURNAL OF APPLIED ANIMAL RESEARCH （ 影响因子：1.63； 五年影响因子：1.727 ）

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收录情况： SCI

摘要： To clone the complete coding sequence of the porcine MEF2a gene and to analyze its expression characterization, two isoforms of MEF2a gene (MEF2aA, MEF2aB) were obtained. Amino acid homology of two isoforms was 86.79%. It was completely identical in 1st -77th and 132th -470th amino acid, and was hypervariable in 78th-131st amino acid. MEF2aB had 34 amino acids (102bp) deletion in 224th-258th amino acid compared with MEF2aA. MEF2a was a broad-spectrum expression gene. Porcine MEF2aA, horse and house mouse existed in the same group, but porcine MEF2aB, cattle and human existed in another group in the phylogenetic tree. Therefore, MEF2a gene was presumed to be an apparent variation during evolutionary process.

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