Cloning and characterization of two novel crystal protein genes, cry54Aa1 and cry30Fa1, from Bacillus thuringiensis strain BtMC28
文献类型： 外文期刊
作者： Tan, Furong 1 ; Zhu, Jun 1 ; Tang, Jie 1 ; Tang, Xueming 2 ; Wang, Shiquan 1 ; Zheng, Aiping 1 ; Li, Ping 1 ;
作者机构： 1.Sichuan Agr Univ, Rice Res Inst, Yaan, Peoples R China
2.Shanghai Acad Agr Sci, Biotechnol Res Ctr, Shanghai, Peoples R China
期刊名称：CURRENT MICROBIOLOGY （ 影响因子：2.188； 五年影响因子：2.197 ）
ISSN：
年卷期：
页码：
收录情况： SCI
摘要： Bacillus thuringiensis strain BtMC28 was isolated from the soil sample in China. Two novel crystal protein genes were found by using the PCR-RFLP method. Moreover, the full-length sequences of two novel genes were obtained by a single oligonucleotide nested (SON)-PCR upstream and downstream strategy. Sequence analysis revealed that one gene encoded a polypeptide of 673 amino acid residues with a molecular mass of 76.3 kDa, 38% identical to Cry10Aa, and the other encoded a polypeptide of 687 amino acid residues with a molecular mass of 77.1 kDa, 74% identical to Cry30Aa. These two novel crystal protein genes were designated as cry54Aa1 and cry30Fa1 by Bt Insecticidal Crystal Proteins Nomenclature Committee, respectively. The Cry54Aa1 and Cry30Fa1 proteins retained five conserved regions commonly found in the existing Cry proteins. Cry54Aa1 protein exhibited insecticidal activities against Laphygma exigua (Lepidoptera), Helicoverpa armigera (Lepidoptera), and Aedes aegypti (Diptera) when its encoding gene was expressed in an Escherichia coli host strain.
- 相关文献
作者其他论文 更多>>
-
Physiological, transcriptomic, and metabolomic analyses reveal that Pantoea sp. YSD J2 inoculation improves the accumulation of flavonoids in Cyperus esculentus L. var. sativus
作者：Wang, Saisai;Huang, Yanna;Tang, Xueming;Wang, Saisai;Huang, Yanna;Tang, Xueming;Wang, Saisai;Huang, Yanna;Tang, Xueming;Sun, Yu;Wang, Jinbin
关键词：Tigernut; Plant growth-promoting microorganisms; Flavonoid biosynthesis; Metabolome; Transcriptome
-
A rapid and high-throughput system for the detection of transgenic products based on LAMP-CRISPR-Cas12a
作者：Liu, Hua;Zeng, Haijuan;He, Chuan;Wang, Jinbin;Cheng, Fang;Tang, Xueming;Tang, Xueming
关键词：GM organisms; CRISPR-Cas12a; LAMP; Rapid detection
-
A Rapid and Visual Method for Nucleic Acid Detection of Escherichia coli O157:H7 Based on CRISPR/Cas12a-PMNT
作者：Jiang, Wei;He, Chuan;Bai, Lan;Chen, Yifan;Jia, Junwei;Lv, Beibei;Tang, Xueming;Wu, Xiao;Jiang, Wei;He, Chuan;Bai, Lan;Chen, Yifan;Pan, Aihu;Lv, Beibei;Tang, Xueming;Wu, Xiao;Jiang, Wei;He, Chuan;Bai, Lan;Chen, Yifan;Jia, Junwei;Pan, Aihu;Lv, Beibei;Tang, Xueming;Wu, Xiao;Tang, Xueming
关键词：foodborne pathogenic bacteria; Escherichia coli O157; H7; nucleic acid detection; POCT; visual method; CRISPR-Cas12a; PMNT
-
A rapid and high-throughput Helicobacterpylori RPA-CRISPR/Cas12a-based nucleic acid detection system
作者：Liu, Hua;Zhang, Chao;Wang, Jinbin;Hu, Xiuwen;Tang, Xueming
关键词：Helicobacter pylori; UreB gene; RPA-CRISPR; Cas12a; Rapid high-throughput detection
-
Screening and production of dsRNA molecules for protecting Cucumis sativus against Cucumber mosaic virus through foliar application
作者：Mo, Qin;Lv, Beibei;Sun, Yu;Wu, Xiao;Song, Lili;Tang, Xueming;Cai, Run
关键词：Cucumber mosaic virus; Double-stranded RNA; RNA-mediated virus resistance; Crop protection
-
Isolation, Classification, and Growth-Promoting Effects of Pantoea sp. YSD J2 from the Aboveground Leaves of Cyperus Esculentus L. var. sativus
作者：Wang, Saisai;Tang, Xueming;Wang, Jinbin;Zhou, Yifan;Huang, Yanna;Tang, Xueming;Tang, Xueming;Wang, Jinbin;Zhou, Yifan;Huang, Yanna
关键词：
-
Comparative Analysis on Rhizosphere Soil and Endophytic Microbial Communities of Two Cultivars of Cyperus esculentus L. Var. Sativus
作者：Wang, Saisai;Tang, Xueming;Wang, Jinbin;Zhou, Yifan;Huang, Yanna;Tang, Xueming;Wang, Jinbin;Zhou, Yifan;Huang, Yanna;Tang, Xueming
关键词：Cyperus esculentus L.; Plant compartments; Microbial community composition; Microbial diversity; 16 s rRNA and ITS amplicon sequencing
