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Dietary supplementation of thiamine enhances colonic integrity and modulates mucosal inflammation injury in goats challenged by lipopolysaccharide and low pH

文献类型： 外文期刊

作者： Ma, Yi 1 ; Wang, Chao 2 ; Elmhadi, Mawda 1 ; Zhang, Hao 1 ; Liu, Fuyuan 3 ; Gao, Xingliang 3 ; Wang, Hongrong 1 ;

作者机构： 1.Yangzhou Univ, Coll Anim Sci & Technol, Lab Metab Manipulat Herbivorous Anim Nutr, Yangzhou 225009, Jiangsu, Peoples R China

2.Univ Western Australia, Sch Biomed Sci, Queen Elizabeth II Med Ctr, Nedlands, WA, Australia

3.Xinjiang Acad Agr & Reclamat Sci, State Key Lab Sheep Genet Improvement & Hlth Prod, Shihezi, Peoples R China

关键词： Thiamine; Colonic integrity; Inflammation; Apoptosis; High-concentrate diet; Goat

期刊名称：BRITISH JOURNAL OF NUTRITION （ 影响因子：4.125； 五年影响因子：4.862 ）

ISSN： 0007-1145

年卷期：

页码：

收录情况： SCI

摘要： The current study aimed to investigate the protective effects of dietary thiamine supplementation on the regulation of colonic integrity and mucosal inflammation in goats fed a high-concentrate (HC) diet. Twenty-four Boer goats (live weight of 35 center dot 62 (sem 2 center dot 4) kg) were allocated to three groups (CON: concentrate/forage = 30:70; HC; concentrate/forage = 70:30 and HCT: concentrate/forage = 70:30 with 200 mg thiamine/kg DMI) for 12 weeks. Results showed that compared with the HC treatment, the HCT group had a significantly higher ruminal pH value from 0 to 12 h after the feeding. The haematoxylin-eosin staining showed that desquamation and severe cellular damage were observed in the colon epithelium of the HC group, whereas the HCT group exhibited more structural integrity of the epithelial cell morphology. Compared with the HC treatment, the HCT group showed a markedly increase in pyruvate dehydrogenase and alpha-ketoglutarate dehydrogenase enzymes activity. The mRNA expressions in the colonic epithelium of SLC19A2, SLC19A3, SLC25A19, Bcl-2, occludin, claudin-1, claudin-4 and ZO-1 in the HCT group were significantly increased in comparison with the HC diet treatment. Compared with the HC treatment, the HCT diet significantly increased the protein expression of claudin-1 and significantly decreased the protein expression of NF-kappa B-related proteins p65. The results show that dietary thiamine supplementation could improve the colon epithelial barrier function and alleviate mucosal inflammation injury in goats after lipopolysaccharide and low pH challenge.

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