您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

A Cytochrome P450 Monooxygenase in Nondefoliating Strain of Verticillium dahliae Manipulates Virulence via Scavenging Reactive Oxygen Species

文献类型： 外文期刊

作者： Zhang, Jiayi 1 ; Jin, Xianjiang 1 ; Wang, Yonglin 1 ; Zhang, Baolong 2 ; Liu, Tingli 2 ;

作者机构： 1.Beijing Forestry Univ, Coll Forestry, Beijing Key Lab Forest Pest Control, Beijing 100083, Peoples R China

2.Jiangsu Acad Agr Sci, Excellence & Innovat Ctr, Nanjing 210014, Jiangsu, Peoples R China

关键词： cytochrome P450; fungal pathogens; host-parasite interactions; microbe-genome sequencing; Verticillium dahliae; virulence

期刊名称：PHYTOPATHOLOGY （ 影响因子：4.01； 五年影响因子：4.457 ）

ISSN： 0031-949X

年卷期： 2022 年 112 卷 8 期

页码：

收录情况： SCI

摘要： Verticillium dahliae is a broad host-range phytopathogenic fungus that causes destructive vascular wilt on plants worldwide. Cytochrome P450 monooxygenases, also known as CYPs/P450s, are broadly distributed in organisms and are involved in a diverse array of molecular/metabolic processes. In this study, using reverse transcription quantitative PCR analysis, we observed that the expression of a P450 gene (Chr2g00380) in the E-class P450, group IV from V. dahliae isolate JR2 was highly induced during tomato infection. Targeted deletion of Chr2g00380 in JR2 did not affect hyphal growth and morphology; however, the mutants exhibited increased sensitivity to H2O2 and defects in melanized microsclerotia formation compared with the wild type. Loss of Chr2g00380 resulted in reduced virulence on tomato and tobacco plants but did not cause phenotypic changes in infection structure formation or in the penetration of cellophane membranes. These data provide evidence for an involvement of a cytochrome P450 monooxygenase in virulence in V. dahliae.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台