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Highly Prevalent Multidrug-Resistant Campylobacter spp. Isolated From a Yellow-Feathered Broiler Slaughterhouse in South China

文献类型： 外文期刊

作者： Bai, Jie 1 ; Chen, Zhengquan 1 ; Luo, Kaijian 1 ; Zeng, Fanliang 1 ; Qu, Xiaoyun 1 ; Zhang, Hongxia 1 ; Chen, Kaifeng; 1 ;

作者机构： 1.South China Agr Univ, Coll Vet Med,Natl & Reg Joint & Engn Lab, Minist Agr,Key Lab Zoonoses,Guangdong Lab Lingnan, Key Lab Zoonoses Prevent & Control Guangdong Prov, Guangzhou, Peoples R China

2.Guangdong Acad Agr Sci, Inst Anim Hlth, Minist Agr,Sci Observat & Expt Stn Vet Drugs & Di, Key Lab Livestock Dis Prevent Guangdong Prov, Guangzhou, Peoples R China

关键词： multidrug-resistant Campylobacter; yellow broiler; slaughtering line; virulence genes; pulse field gel electrophoresis

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.64； 五年影响因子：6.32 ）

ISSN： 1664-302X

年卷期： 2021 年 12 卷

页码：

收录情况： SCI

摘要： The purpose of this study was to investigate the prevalence, antimicrobial resistance, virulence genes, and genetic diversity of Campylobacter spp. along the yellow-feathered broiler slaughtering line in Southern China from December 2018 to June 2019. A total of 157 Campylobacter spp. isolates were identified from 1,102 samples (including 53.6% (75/140) of live chicken anal swab samples, 27.5% (44/160) of defeathering samples, 18.1% (29/160) of evisceration samples, 2.1% (3/140) of washing samples, 1.4% (2/140) of chilling samples, and 1.1% (4/362) of environmental samples). The prevalence of Campylobacter spp. was 14.2%, including 43.9% Campylobacter jejuni, 53.5% Campylobacter coli, and 2.5% other Campylobacter species. The highest antimicrobial resistance rate was found to be against sulfamethoxazole (138/157, 87.9%), and 90.4% (142/157) of the isolates were multidrug resistant (MDR). Examination of resistance-related genes revealed the double base mutated Thr-86-Ile, which informed ACA-TTA, with an Arg-79-Lys substitution in gyrA. Eleven virulence-associated genes (cadF, cdtA, cdtB, ciaB, flaA, imaA, dnaJ, plaA, virB11, racR, and cdtC) were also detected by a polymerase chain reaction (PCR) analysis, and cadF (81.5%) was the most prevalent. Based on an analysis of pulsed-field gel electrophoresis (PFGE) results, we found that Campylobacter spp. could be cross-contaminated throughout the entire slaughtering line. These results show that it is imperative to study the Campylobacter spp. from the yellow-feathered broiler along the slaughtering line in China to develop preventative and treatment measures for the poultry industry, as well as food safety and public health.

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