Influence of PepF peptidase and sporulation on microcin J25 production in Bacillus subtilis
文献类型： 外文期刊
作者： Zhang, Guangwen 1 ; Feng, Saixiang 1 ; Qin, Miaomiao 1 ; Sun, Juan 1 ; Liu, Yutong 1 ; Luo, Changqi 1 ; Lin, Min 1 ; Xu, Siqi 1 ; Liao, Ming 1 ; Fan, Huiying 1 ; Liang, Zhaoping 1 ;
作者机构： 1.South China Agr Univ, Coll Vet Med, Guangzhou, Peoples R China
2.Key Lab Zoonosis Prevent & Control Guangdong Prov, Guangzhou, Peoples R China
3.South China Agr Univ, Guangdong Lab Lingnan Modern Agr, Guangzhou, Peoples R China
4.Minist Agr, Key Lab Vet Vaccine Innovat, Guangzhou, Peoples R China
5.Natl & Reg Joint Engn Lab Medicament Zoonosis Prev, Guangzhou, Peoples R China
6.Guangdong Acad Agr Sci, Inst Anim Hlth, Guangzhou, Peoples R China
关键词： microcin J25; peptidase; PepF; Bacillus subtilis; sporulation
期刊名称：MICROBIOLOGY SPECTRUM （ 影响因子：3.7； 五年影响因子：5.9 ）
ISSN： 2165-0497
年卷期： 2024 年
页码：
收录情况： SCI
摘要： The lasso peptide microcin J25 (MccJ25) possesses strong antibacterial properties and is considered a potential effective component of bacterial disease treatment drugs and safe food preservatives. Although MccJ25 can be heterologously expressed in Bacillus subtilis as we have previously reported, its regulation and accumulation are yet to be understood. Here, we investigated the expression level and stability of MccJ25 in B. subtilis strains with disruption in peptidase genes pepA, pepF, and pepT. Oligoendopeptidase F (PepF) was found to be involved in reduction of the production of MccJ25 by degradation of its precursor peptide. In the pepF mutant, the MccJ25 reached a concentration of 1.68 mu M after a cultivation time exceeding 60 hours, while the wild-type strain exhibited a concentration of only 0.14 mu M. Moreover, the production of MccJ25 in B. subtilis downregulated the genes associated with sporulation, and this may contribute to its accumulation. Finally, this study provides a strategy to improve the stability and production of MccJ25 in B. subtilis.
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