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Molecular cloning, characterization and expression analysis of a clip-domain serine protease from pearl oyster Pinctada fucata

文献类型： 外文期刊

作者： Zhang, Dianchang 3 ; Jiang, Shigui 3 ; Ma, Jianjun 3 ; Jiang, Jingjing 3 ; Pan, Dequan 3 ; Xu, Xinping 1 ;

作者机构： 1.Sun Yat Sen Univ, Sch Life Sci, Lab Biocontrol, Minist Educ, Guangzhou 510275, Guangdong, Peoples R China

2.Sun Yat Sen Univ, Sch Life Sci, Key Lab Gene Engn, Minist Educ, Guangzhou 510275, Guangdong, Peoples R China

3.Chinese Acad Fishery Sci, S China Sea Fisheries Res Inst, Div Aquaculture & Biotechnol, Guangzhou 510300, Guangdong, Peoples R China

4.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 200090, Peoples R China

5.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shangha

关键词： Serine proteinase;Polyadenylation;Open Serine proteinase;Open reading frames;mRNA;Computer programs;Immune response;Gonads;Mantle;software;Hemocytes;Signal transduction;Gills;Amino acid sequence;Acute phase substances;Glands;3' Untranslated regions;Lipopolysaccharides;Homology;Adductor muscle;Embryogenesis;Isoelectric points;5' Untranslated Regions;Nucleotides;Pearl oysters;Nucleotide sequence;Digestive glands;Blood cells;Immunity;DNA;Pathogenic bacteria;Pinctada fucata;Vibrio alginolyticus

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

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收录情况： SCI

摘要： The clip-domain serine proteases (SPs) are the essential components of extracellular signaling cascade in various biological processes, especially in embryonic development and the innate immune responses of invertebrate. Herein, we described the isolation and characterization of pearl oyster Pinctada fucata clip-domain SP gene (designated as poSP). The poSP cDNA was 1080 bp long and consisted of a 5'-untranslated region (UTR) of 13 bp, a 3'-UTR of 68 bp with a polyadenylation signal (AATAAA) at 22 nucleotides upstream of the poly(A) tail, and an open reading frame (ORF) of 999 bp encoding a polypeptide of 332 amino acids with an estimated molecular mass of 36.5 kDa and a theoretical isoelectric point of 7.3. A clip-domain and a trypsin-like serine protease domain were identified in the poSP using SMART analysis. Homology analysis of the deduced amino acid sequence of the poSP with other known SP sequences by MatGAT software revealed that the poSP shared 47.0-68.4% similarity to the other known SP sequences. The poSP mRNA was expressed in haemocytes, gonad, digestive gland and mantle, but not expressed in adductor muscle and gill. The poSP mRNA was up-regulated and increased nearly double-fold after LPS or Vibrio alginolyticus stimulation, respectively. These results suggested that the poSP was an inducible acute-phase protein that perhaps involved in the innate immune response of pearl oyster.

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