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Characterization, expression, and functional study of IRAK-1 from grouper, Epinephelus coioides

文献类型： 外文期刊

作者： Li, Yan-Wei 1 ; Zhao, Fei 3 ; Mo, Ze-Quan 1 ; Luo, Xiao-Chun 4 ; Li, An-Xing 2 ; Dan, Xue-Ming 1 ;

作者机构： 1.South China Agr Univ, Coll Marine Sci, 483 Wushan St, Guangzhou 510642, Guangdong, Peoples R China

2.Sun Yat Sen Univ, Sch Life Sci, State Key Lab Biocontrol, 135 Xingang West St, Guangzhou 510275, Guangdong, Peoples R China

3.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Fishery Drug Dev, Minist Agr, Guangzhou 510380, Guangdong, Peoples R China

4.South China Univ Technol, Sch Biosci & Biotechnol, Guangzhou 510006, Guangdong, Peoples R China

关键词： Epinephelus coioides; IRAK-1; Signal transduction; Cryptocaryon irritans

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

ISSN： 1050-4648

年卷期： 2016 年 56 卷

页码：

收录情况： SCI

摘要： As crucial components of the toll-like receptor (TLR) and interleukin-1 (IL-1) receptor (IL-1R) signaling pathways, interleukin-1 receptor associated kinase (IRAK) family members play essential roles in an animal's immune response. In this study, an IRAK family member, designated EcIRAK-1, was identified in the orange-spotted grouper Epinephelus coioides, and its role in signal transduction investigated. The full-length EcIRAK-1 gene is 2822 bp, encoding a 760-amino-acid protein that has the typical characteristics of mammalian IRAK-1, including an N-terminal death domain, a ProST domain, a central kinase domain, and C-terminal Cl and C2 domains. EcIRAK-1 shares 42%-79% sequence identity with other fish IRAK-1 proteins, and the death and kinase domains are more conserved than the other domains. Several important amino acids and motifs of mammalian IRAK-1 are also conserved in the grouper and other piscine IRAK-1s. In healthy grouper, EcIRAK-1 was broadly expressed in all the tissues tested, with the highest expression in the gill and skin. After infection with Cryptocaryon irritans, EcIRAK-1 expression increased in the gill and spleen. After its exogenous expression in HEK293T cells, EcIRAK-1 significantly activated nuclear factor kappaB (NF-KB). The death domain, ProST domain, and some conserved amino acids, such as T58, T207, K237, and T387, in EcIRAK-1 are required for its signaling function. These data demonstrate that piscine IRAK-1 has the same structural characteristics as its mammalian counterpart and that its function is conserved among vertebrates. (C) 2016 Published by Elsevier Ltd.

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