Transcriptomic profiling revealed the signatures of intestinal barrier alteration and pathogen entry in turbot (Scophthalmus maximus) following Vibrio anguillarum challenge
文献类型： 外文期刊
作者： Gao, Chengbin 1 ; Fu, Qiang 2 ; Su, Baofeng 3 ; Zhou, Shun 1 ; Liu, Fengqiao 1 ; Song, Lin 1 ; Zhang, Min 1 ; Ren, Yichao 1 ;
作者机构： 1.Qingdao Agr Univ, Marine Sci & Engn Coll, Qingdao 266109, Peoples R China
2.East China Normal Univ, State Key Lab Estuarine & Coastal Res, Shanghai 200062, Peoples R China
3.Chinese Acad Fishery Sci, Heilongjiang Fisheries Res Inst, Key Lab Freshwater Aquat Biotechnol & Breeding, Minist Agr, Harbin 150070, Peoples R China
4.Chinese Acad Fishery Sci, Heilongjiang Fisheries Res Inst, Natl & Local Joint Engn Lab Freshwater Fish Breed, Harbin 150070, Peoples R China
5.Qingdao Agr Univ, Sch Int Educ & Exchange, Qingdao 266109, Peoples R China
关键词： Turbot; RNA-seq; Intestine; Immune response; Vibrio anguillarum
期刊名称：DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY （ 影响因子：3.636； 五年影响因子：3.654 ）
ISSN： 0145-305X
年卷期： 2016 年 65 卷
页码：
收录情况： SCI
摘要： The mucosal immune system serves as the frontline barriers of host defense against pathogen infection, especially for the fishes, which are living in the pathogen rich aquatic environment. The intestine constitutes the largest surface body area in constantly contact with the external pathogens, and plays a vital role in the immune defense against inflammation and pathogen infection. Previous studies have revealed that fish intestine might serves as the portal of entry for Vibrio anguillarum. To characterize the immune actors and their associated immune activities in turbot intestine barrier during bacterial infection, here we examined the gene expression profiles of turbot intestine at three time points following experimental infection with V. anguillarum utilizing RNA-seq technology. A total of 122 million reads were assembled into 183,101 contigs with an average length of 1151 bp and the N50 size of 2302 bp. Analysis of differential gene expression between control and infected samples at 1 h, 4 h, and 12 h revealed 2079 significantly expressed genes. Enrichment and pathway analysis of the differentially expressed genes showed the centrality of the pathogen attachment and recognition, antioxidant/apoptosis, mucus barrier modification and immune activation/inflammation in the pathogen entry and host immune responses. The present study reported the novel gene expression patterns in turbot mucosal immunity, which were overlooked in previous studies. Our results can help to understand the mechanisms of turbot host defense, and may also provide foundation to identify the biomarkers for future selection of disease resistant broodstock and evaluation of disease prevention and treatment options. (C) 2016 Elsevier Ltd. All rights reserved.
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