Identification and expression analysis of toll-like receptor genes (TLR8 and TLR9) in mucosal tissues of turbot (Scophthalmus maximus L.) following bacterial challenge
文献类型： 外文期刊
作者： Dong, Xiaoyu 1 ; Su, Baofeng 2 ; Zhou, Shun 1 ; Shang, Mei 2 ; Yan, Hao 2 ; Liu, Fengqiao 1 ; Gao, Chengbin 1 ; Tan, Feng 1 ;
作者机构： 1.Qingdao Agr Univ, Marine Sci & Engn Coll, Qingdao 266109, Peoples R China
2.Chinese Acad Fishery Sci, Heilongjiang Fisheries Res Inst, Minist Agr, Key Lab Freshwater Aquat Biotechnol & Breeding, Harbin 150070, Peoples R China
3.Chinese Acad Fishery Sci, Heilongjiang Fisheries Res Inst, Natl & Local Joint Engn Lab Freshwater Fish Breed, Harbin 150070, Peoples R China
4.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China
5.Qingdao Agr Univ, Sch Int Educ & Exchange, Qingdao 266109, Peoples R China
关键词： TLR8; TLR9; Mucosal tissues; Scophthalmus maximus; Vibrio anguillarum; Streptococcus iniae
期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）
ISSN： 1050-4648
年卷期： 2016 年 58 卷
页码：
收录情况： SCI
摘要： Mucosal immune system is one of the most important components in the innate immunity and constitutes the front line of host defense against infection, especially for teleost, which are living in the pathogen-rich aquatic environment. The pathogen recognition receptors (PRRs), which can recognize the conserved pathogen-associated molecular patterns (PAMPs) of bacteria, are considered as one of the most important component for pathogen recognition and immune signaling pathways activation in mucosal immunity. In this regard, we sought to identify TLR8 and TLR9 in turbot (Scophthalmus maximus), as well as their mucosal expression patterns following different bacterial infection in mucosal tissues for the first time. The full-length TLR8 transcript consists of an open reading frame (ORF) of 3108 bp encoding the putative peptide of 1035 amino acids. While the TLR9 was 6730 bp long, containing a 3168 bp ORF that encodes 1055 amino acids. The phylogenetic analysis revealed both TLR8 and TLR9 showed the closest relationship to large yellow croaker. Moreover, both TLR8 and TLR9 could be detected in all examined healthy turbot tissues, with the lowest expression level in liver and a relatively moderate expression pattern in healthy mucosal tissues. Distinct expression patterns of TLR8 and TLR9 were comparatively observed in the mucosal tissues (intestine, gill and sldn) following Vibrio anguillarum and Streptococcus iniae infection, suggesting their different roles for mucosal immunity. Further functional studies are needed to better characterize TLR8 and TLR9 and their family members, to better understand the ligand specificity and to identify their roles in different mucosal tissues in protecting fish from the pathogenically hostile environment. (C) 2016 Elsevier Ltd. All rights reserved.
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