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The expression signatures of neuronal nitric oxide synthase (NOS1) in turbot (Scophthalmus maximus L.) mucosal surfaces against bacterial challenge

文献类型： 外文期刊

作者： Dong, Xiaoyu 1 ; Fu, Qiang 5 ; Liu, Song 6 ; Gao, Chengbin 1 ; Su, Baofeng 2 ; Tan, Fenghua 4 ; Li, Chao 1 ;

作者机构： 1.Qingdao Agr Univ, Marine Sci & Engn Coll, Qingdao 266109, Peoples R China

2.Chinese Acad Fishery Sci, Heilongjiang Fisheries Res Inst, Natl & Local Joint Engn Lab Freshwater Fish Breed, Harbin 150070, Peoples R China

3.Chinese Acad Fishery Sci, Heilongjiang Fisheries Res Inst, Key Lab Freshwater Aquat Biotechnol & Breeding, Minist Agr, Harbin 150070, Peoples R China

4.Qingdao Agr Univ, Sch Int Educ & Exchange, Qingdao 266109, Peoples R China

5.China Normal Univ, State Key Lab Estuarine & Coastal Res, Shanghai 200062, Peoples R China

6.Funct Zone Coordinating Off Huangdao Dist, West Coast New Area, Qingdao 266555, Peoples R China

关键词： Neuronal nitric oxide synthase (NOS1); Turbot; Mucosal immunity; Vibrio anguillarum; Streptococcus iniae

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

ISSN： 1050-4648

年卷期： 2016 年 59 卷

页码：

收录情况： SCI

摘要： The mucosal surfaces constitute the first immune barrier of host defense and also serve as the dynamic interfaces that simultaneously mediate a diverse array of critical physiological processes. It has been long hypothesized that observed difference of disease resistance among different fish strains and species are strongly correlated to the activities of the immune actors in mucosal surfaces. Particularly, neuronal NOS (nNOS or NOS1) is a constitutively expressed gene that catalyzes the oxidation of L-arginine and water to nitric oxide (NO), which is known as a potent host defence effector in immune system with antimicrobial activity. Moreover, NOS1 was detected to be expressed in fish mucosal surfaces, but its activities in mucosal immune responses were always overlooked. In this regard, we identified the NOS1 of turbot and characterized its expression patterns in mucosal tissues following Vibrio anguillarum and Streptococcus iniae challenge. The results showed that the NOS1 gene had a 4389 bp open reading frame (ORF) that encoded 1462 amino acids. Phylogenetic analysis showed the turbot NOS1 had the strongest relationship to Larimichthys crocea. And the syntenic analysis revealed the similar neighboring genes associated with turbot NOS1, compared with other teleost and mammals. In addition, NOS1 was widely expressed in all examined tissues with the highest expression level in brain, followed by intestine and gill. Finally, the NOS1 showed a general trend of up -regulation in mucosal tissues following both bacterial challenge, with the highest up -regulation in intestine. The significant quick induction of NOS1 in mucosal surfaces against infection indicated its key roles to prevent pathogen attachment and entry in mucosal immunity. More functional studies are needed to conduct in teleost to better understand the roles of NOS1 in maintaining the integrity of the mucosal barriers against infection. (C) 2016 Elsevier Ltd. All rights reserved.

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