HSPC117 Improves the Rate of Embryonic Development by Up-Regulating the Transcription of Proto-Oncogenes
文献类型： 外文期刊
作者： Ma, Hong 1 ; Fu, Bo 1 ; Wang, Liang 1 ; Li, Zhong-Qiu 1 ; Liu, Di 1 ;
作者机构： 1.Heilongjiang Acad Agr Sci, Harbin 150086, Heilongjiang, Peoples R China
关键词： HSPC117; Porcine; Embryonic development; Proto-oncogenes; Epigenetics
期刊名称：PAKISTAN JOURNAL OF ZOOLOGY （ 影响因子：0.831； 五年影响因子：0.768 ）
ISSN： 0030-9923
年卷期： 2019 年 51 卷 5 期
页码：
收录情况： SCI
摘要： The developmental efficiency of cloned embryos from somatic cells remains low, and many factors contribute to overall development of the embryo, including gene expression by the donor cells. The human hematopoietic stem/progenitor cell 117 (HSPC117) protein has been identified as being involved in placental formation, and can be modified by epigenetics. However, whether HSPC117 affects development of cloned embryos mRNA expression is unknown. To investigate the influences of HSPC117 on embryonic development, we generated transgenic porcine embryos by handmade cloning. We then assessed the embryonic developmental rate at cleavage and blastocyst stages. Our results showed that the HSPC117 transgenic embryos had markedly higher cleavage and blastocyst rates when compared to the embryos with pcDNA 3.1 vector, (69.7 +/- 3.5% vs. 64.6 +/- 1.8%, and 24.8 +/- 2.2% vs. 15.9 +/- 4.3%, respectively). However, blastocyst cell number was not different between groups. Furthermore, proto-oncogene was reported to play roles in embryo development, thus we assessed c-Fos, c-Jun, Raf-1, and c-Myc mRNA expression in HSPC117 transgenic and pcDNA 3.1 blastocysts. It was revealed that over-expression of HSPC117 mRNA in blastocysts up-regulated c-Fos, c-Jun, Raf-1, and c-Myc mRNA expression. We suggest that over-expressed HSPC117 is an important contributing factor to the development of HMC embryos in vitro via the regulation of mRNA expression of several proto-oncogenes.
- 相关文献
作者其他论文 更多>>
-
PABPN1 functions as a downstream gene of CREB to inhibit the proliferation of preadipocytes
作者：Zhang, Xiao-Han;Li, Jia-Xin;Wu, Xiao-Xu;Zhang, Qian;Yang, Si-Qi;Yang, Xiu-Qin;Tian, Ming;Liu, Di
关键词：CREB; PABPN1; Preadipocyte; Proliferation; Regulatory Axis
-
Two telomere-to-telomere pig genome assemblies and pan-genome analyses provide insights into genomic structural landscape and genetic adaptations
作者：Zong, Wencheng;Chen, Li;Wang, Jinbu;Hou, Xinhua;Liu, Xin;Wang, Ligang;Wang, Lixian;Zhang, Longchao;Chen, Li;Chai, Jie;Wang, Jinyong;Zhang, Dongjie;Tian, Ming;He, Xinmiao;Liu, Di;Zhang, Yuebo;He, Jun;Yin, Yulong;Zhang, Yuebo;He, Jun;Yin, Yulong;An, Yalong;Song, Chengyi;D'Alessandro, Enrico;Li, Mingzhou
关键词：
-
Integration of Multiomics Data Reveals Selection Characteristics of ITGB1 That Are Associated with Size Differentiation in Pigs
作者：Wu, Guandong;Yu, Miao;Liu, Tianxin;Chang, Yang;Liu, Zhonghua;Xu, Chunzhu;Zhang, Dongjie;Liu, Di
关键词：Min pig; selection characteristics; transcriptome; resequencing;
ITGB1 -
Identification and functional analysis of a new cold induced LncRNA44154
作者：Zhang, Dongjie;Ma, Shouzheng;Wang, Liang;Liu, Di
关键词：Pig; Adipogenesis; Lipid metabolism; lncRNA44154
-
Aspartate Metabolism-Driven Gut Microbiota Dynamics and RIP-Dependent Mitochondrial Function Counteract Oxidative Stress
作者：Jin, Shunshun;Wang, Chenyu;He, Yiwen;Zhou, Hui;He, Xinmiao;He, Liuqin;Wu, Jian;Wang, Chenyu;He, Yiwen;Tang, Yulong;Huang, Le;Zhou, Hui;Peng, Can;Li, Tiejun;Yin, Yulong;He, Liuqin;Jin, Shunshun;Wu, Ziping;Liu, Di;Feng, Yanzhong;Chen, Heshu;He, Xinmiao;Yang, Guan;Qiu, Jiazhang;Yin, Yulong
关键词：aspartate; microbiota; oxidative stress; RIP pathway
-
Isolation and Characterization of Seneca Valley Virus From Pig Transboundary Spread to the Mink Infection
作者：Qin, Ziliang;Chen, Chao;Chen, Shaojun;Nai, Zida;Li, Gang;Liu, Di;Jiang, Xinpeng;He, Xinmiao;Wang, Wentao;Wang, Fang;Tian, Ming;He, Haijuan;Chen, Heshu;Liu, Di;Wang, Yao
关键词：cross-host transmission; mink; Seneca Valley virus
-
Positive selection on rare variants of IGF1R and BRD4 underlying the cold adaptation of wild boar
作者：Chen, Jianhai;Xu, Zhixiang;Guo, Yapin;Kuang, Renzuo;Zhao, Yunxia;Zhao, Shuhong;Chen, Jianhai;Xia, Shengqian;Jia, Yangying;Chen, Jianhai;Jakovlic, Ivan;Jakovlic, Ivan;Jakovlic, Ivan;Sablin, Mikhail;Zhong, Jie;Tran, Nhien Thuy Thi;Tran, Nhien Thuy Thi;Yang, Hao;Ma, Hong;Liu, Di;Sprem, Nikica;Han, Jianlin;Zhao, Yunxia;Zhao, Shuhong
关键词：
