Optimization of ASE and SPE conditions for the HPLC-FLD detection of piperazine in chicken tissues and pork
文献类型： 外文期刊
作者： Liu, Chujun 1 ; Xie, Xing 3 ; Wang, Bo 2 ; Zhao, Xia 1 ; Guo, Yawen 1 ; Zhang, Yangyang 1 ; Bu, Xiaona 1 ; Xie, Kaizhou 1 ;
作者机构： 1.Yangzhou Univ, Coll Anim Sci & Technol, Yangzhou 225009, Jiangsu, Peoples R China
2.Yangzhou Univ, Joint Int Res Lab Agr & Agriprod Safety, Yangzhou, Jiangsu, Peoples R China
3.Jiangsu Acad Agr Sci, Inst Vet Med, Key Lab Vet Biol Engn & Technol, Minist Agr, Nanjing, Jiangsu, Peoples R China
4.Yangzhou Univ, Coll Vet Med, Yangzhou, Jiangsu, Peoples R China
关键词： ASE; chicken tissues; HPLC-FLD; piperazine; pork; SPE
期刊名称：CHIRALITY （ 影响因子：2.437； 五年影响因子：2.204 ）
ISSN： 0899-0042
年卷期： 2019 年 31 卷 10 期
页码：
收录情况： SCI
摘要： Accelerated solvent extraction (ASE) and solid-phase extraction (SPE) conditions were optimized by a high-performance liquid chromatography-fluorescence detector (HPLC-FLD) method for the detection of piperazine in chicken tissues and pork. Piperazine residues were determined by precolumn derivatization with trimethylamine and dansyl chloride. Samples were extracted with 2% formic acid in acetonitrile using an ASE apparatus and purified using a Strata-X-C SPE column. The monosubstituted product of the reaction of piperazine with dansyl chloride was 1-dansyl piperazine (1-DNS-piperazine). Chromatographic separations were performed on an Athena C-18 column (250 x 4.6 mm, id: 5 mu m) with gradient elution using ultrapure water and acetonitrile (5:95, V/V) as the mobile phase. The calibration curves showed good linearity over a concentration range of LOQ-200.0 mu g/kg with a coefficient of determination (R-2) >= .9992. The recoveries and relative standard deviations (RSD values) ranged from 78.49% to 97.56% and 1.19% to 5.32%, respectively, across the limit of quantification (LOQ) and 0.5, 1, and 2.0 times the maximum residue limit (MRL; mu g/kg). The limits of detection (LODs) and LOQs were 0.96 to 1.85 mu g/kg and 3.20 to 5.50 mu g/kg, respectively. The method was successfully applied for the validation of animal products in the laboratory.
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