您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Enhanced Tolerance of Transgenic Rice Plants Over-Expressing Maize C-4 Phosphoenolpyruvate Carboxylase Gene to Low Nitrogen Conditions

文献类型： 外文期刊

作者： Wei, Xiaodong 1 ; Shi, Dawei 2 ; Li, Xia 1 ; Fang, Xianwen 1 ; Wu, Jiaona 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Food Crops, Nanjing 210014, Jiangsu, Peoples R China

2.Nanjing Forestry Univ, Coll Biol & Environm, Nanjing 210037, Jiangsu, Peoples R China

3.Yangzhou Univ, Jiangsu Coinnovat Ctr Modern Prod Technol Grain C, Yangzhou 225009, Jiangsu, Peoples R China

4.Nanjing Forestry Univ, Coinnovat Ctr Sustainable Forestry Southern China, Nanjing 210037, Jiangsu, Peoples R China

关键词： Phosphoenolpyruvate carboxylase; Transgenic rice; Low nitrogen tolerance; Photosynthesis; Carbon metabolism; Nitrogen metabolism

期刊名称：INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY （ 影响因子：0.822； 五年影响因子：0.906 ）

ISSN： 1560-8530

年卷期： 2019 年 22 卷 4 期

页码：

收录情况： SCI

摘要： The present study identified and compared transgenic rice overexpressing maize C-4 pepc (PC) with wild-type (WT; Kitaake) in low nitrogen tolerance through a combination of physiological, metabolic and proteomic analysis. The results showed that PC had smaller decreases and higher dry weight and net photosynthetic rate under low nitrogen conditions compared with WT. The increased photosynthetic rate in PC was associated with higher phosphoenolpymvate carboxylase (PEPC) activity, pepc gene expression, and antioxidant system enhancement. The transgenic type with PC accumulated less starch or soluble sugars than WT under low nitrogen conditions. Metabolic analysis showed elevated PEPC activity in PC increased the synthesis of malate, citrate and 2-oxoglutarate under low nitrogen conditions. This increase also enhanced enzyme activities of the anaplerotic pathway for the tricarboxylic acid cycle and nitrogen assimilation. Furthermore, leaf N level of PC was higher than WT. Using 2-DE, 12 differentially expressed spots were identified between N deficient PC and WT. The proteins involved in photosynthesis, tricarboxylic acid cycle, amino acid biosynthesis, ROS scavenging, and translation expressed in higher concentration in N deficient PC. The increased carbon flow towards nitrogen metabolism could alleviate the inhibition of photosynthesis and had higher N content in PC under low nitrogen conditions, indicating enhanced tolerance of transgenic type PC to low nitrogen compared with WT. (C) 2019 Friends Science Publishers

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台