Bile salt hydrolase can improve Lactobacillus plantarum survival in gastrointestinal tract by enhancing their adhesion ability
文献类型： 外文期刊
作者： Yang, Yao 1 ; Liu, Yanrong 1 ; Zhou, Sisi 1 ; Huang, Lu 1 ; Chen, Ying 1 ; Huan, Hailin 3 ;
作者机构： 1.Nanjing Normal Univ, Sch Food Sci & Pharmaceut Engn, 1 Wenyuan Rd, Nanjing 210046, Jiangsu, Peoples R China
2.Nanjing Inst Prod Qual Inspect, Natl Ctr Supervis Inspect Processed Food & Food A, 3 Jialingjiang East St, Nanjing 210019, Jiangsu, Peoples R China
3.Jiangsu Acad Agr Sci, Inst Anim Sci, 50 Zhongling St, Nanjing 210014, Jiangsu, Peoples R China
关键词： bile salt hydrolase (BSH); Lactobacillus plantarum; caco-2 cell; adhesion ability; gastrointestinal (GI) survival; lactic acid bacteria (LAB)
期刊名称：FEMS MICROBIOLOGY LETTERS （ 影响因子：2.742； 五年影响因子：2.856 ）
ISSN： 0378-1097
年卷期： 2019 年 366 卷 8 期
页码：
收录情况： SCI
摘要： Lactic acid bacteria (LAB) are major probiotics in food supplements. Survival in gastrointestinal (GI) tract is important for the effective use of LAB as probiotics. Bile salt hydrolase (BSH), which catalyzes the conversion of conjugated bile salts into free bile salts, can significantly modulate the gut microbiome. Here, we hypothesize that BSH is important for LAB survival and adhesion in the gut. The aim of this study is to investigate the effect of BSH on the survival of LAB in the GI tract. A panel of bsh genes from murine gut microbiota were amplified, cloned and expressed into Lactobacillus plantarum, which were then administered to mice by gavage. Our data indicated that the survival of BSH-positive L. plantarum was significantly prolonged in the GI tract compared with wild type L. plantarum. Furthermore, BSH-positive strains exhibited increased adhesion to Caco-2 intestinal cells than BSH-deleted L. plantarum. Enhanced adhesion to intestinal cells of BSH positive LAB can therefore be an important criterion for selecting effective probiotic strains in food industry.
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