Recent developments in heterologous production of cellulases and xylanases using the Pichia pastoris expression system
文献类型： 外文期刊
作者： Ullah, Hayat 1 ; Madadi, Meysam 1 ; Asadollahi, Mohammad Ali 2 ; Hu, Yun 1 ; Dou, Shaohua 3 ; Yan, Junshu 4 ; Huan, Hailin 4 ; Sun, Fubao 1 ;
作者机构： 1.Jiangnan Univ, Sch Biotechnol, Key Lab Ind Biotechnol, Minist Educ, Wuxi, Peoples R China
2.Univ Isfahan, Fac Biol Sci & Technol, Dept Biotechnol, Esfahan, Iran
3.Dalian Univ, Coll Life & Hlth, Dalian, Peoples R China
4.Jiangsu Acad Agr Sci, Inst Anim Sci, Nanjing, Peoples R China
5.Jiangnan Univ, Sch Biotechnol, Key Lab Ind Biotechnol, Minist Educ, Wuxi 214122, Peoples R China
关键词： cellulase; CRISPR/Cas9; genetic engineering strategies; P. pastoris; AOX1 promoter; xylanase
期刊名称：JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY （ 影响因子：3.4； 五年影响因子：3.2 ）
ISSN： 0268-2575
年卷期： 2024 年 99 卷 3 期
页码：
收录情况： SCI
摘要： The yeast expression system, Pichia pastoris, is one of the most robust and versatile expression systems in biotechnology and molecular biology, generally considered as a safe host for heterologous protein expression, especially for producing cellulases and xylanases at the industrial level. Despite the high recombinant protein expression rate, the potential of the P. pastoris expression system has still not been fully explored. Cultivation of P. pastoris under optimized conditions greatly relies on the strain and is associated with certain problems such as promoter strength, sensitivity to methanol, and oxygen demand. To address these issues, different genetic engineering strategies have been employed. Advancements in promoter engineering, optimization of gene dosage and codon usage, recombinant plasmid engineering using CRISPR/Cas9 system, and directed evolution strategies have proven beneficial to the yield of cellulase expression levels. This study will systemically review recent progress in various genetic engineering strategies to enhance cellulase and xylanase expression in the P. pastoris expression system. The utilization of alcohol oxidase 1 promoter (pAOX1), methanol-free system, and recombinant plasmid engineering for improved production of these enzymes are highlighted. Additionally, we discuss the recent advancements in the P. pastoris expression system toolbox for improved cellulase and xylanase production, thus providing a deep insight into how P. pastoris is becoming the indispensable platform for heterologous protein production. (c) 2023 Society of Chemical Industry (SCI).
- 相关文献
作者其他论文 更多>>
-
Enhanced enzymatic hydrolysis of lignocellulosic substrate with less-expensive formulation of homemade cellulase cocktails
作者：Liu, Qiangqiang;Sun, Chihe;Madadi, Meysam;Sun, Fubao;Dou, Shaohua;Yan, Junshu;Huan, Hailin;Samimi, Abdolreza;Ashori, Alireza
关键词：Lignocellulosic biomass; High-solid saccharification; Surfactants mixture; Accessory enzymes; Non-catalytic protein; Synergistic effect
-
Dual assistance of surfactants in glycerol organosolv pretreatment and enzymatic hydrolysis of lignocellulosic biomass for bioethanol production
作者：Song, Guojie;Sun, Chihe;Madadi, Meysam;Sun, Fubao;Dou, Shaohua;Yan, Junshu;Huan, Hailin;Aghbashlo, Mortaza;Tabatabaei, Meisam;Tabatabaei, Meisam;Ashori, Alireza
关键词：Sugarcane bagasse; Surfactant-assisted pretreatment; High-solid enzymatic hydrolysis; Cellulosic ethanol; Economic analysis
-
Hydrophobic modification improves the delivery of cell-penetrating peptides to eliminate intracellular pathogens in animals
作者：Tang, Qi;Tan, Peng;Dai, Zhaolai;Jin, Junqi;Zhang, Xin;Zhang, Yucheng;Zhou, Chenlong;Yue, Zitian;Fu, Huiyang;Ma, Xi;Wang, Tao;Ding, Yakun;Yan, Junshu;Ma, Xi
关键词：Intracellular targeting; Cell penetrating peptide; Antimicrobial peptide; Intracellular pathogens; Action mechanism
-
Bacterial lipopolysaccharide with different administration routes affects intestinal mucosal morphological, immunological, and microbial barrier functions in goslings
作者：Xi, Yumeng;Li, Yue;Ying, Shijia;Yan, Junshu;Shi, Zhendan
关键词：gosling; lipopolysaccharide challenge; intestinal mucosal barrier function
-
The effects of dietary protein and fiber levels on growth performance, gout occurrence, intestinal microbial communities, and immunoregulation in the gut-kidney axis of goslings
作者：Xi, Yumeng;Li, Yue;Yan, Junshu;Shi, Zhendan;Huang, Yuanpi;Huang, Yunmao
关键词：crude fiber; protein; gosling gout; intestinal microbial communities; gut-kidney axis
-
Effects of Manure Removal Frequencies and Deodorants on Ammonia and GHG Concentrations in Livestock House
作者：Zhang, Xia;Li, Jian;Shao, Le;Huan, Hailin;Qin, Feng;Zhai, Pin;Yang, Jie;Pan, Xiaoqing;Zhang, Xia;Li, Jian;Shao, Le;Huan, Hailin;Qin, Feng;Zhai, Pin;Yang, Jie;Pan, Xiaoqing
关键词：ammonia; greenhouse gas; manure remove frequency; microbial deodorant; VenaZn deodorant
-
Effects of resveratrol and its derivative pterostilbene on hepatic injury and immunological stress of weaned piglets challenged with lipopolysaccharide
作者：Li, Yue;Tu, Feng;Cao, Jing;Yan, Junshu;Li, Yue;Zhang, Hao;Chen, Yanan;Hou, Xiang
关键词：hepatic injury; immunological stress; lipopolysaccharide; piglet; pterostilbene; resveratrol
