In-depth recognition of mixed surfactants maintaining the enzymatic activity of cellulases through stabilization of their spatial structures
文献类型： 外文期刊
作者： Liu, Qiangqiang 1 ; Madadi, Meysam 1 ; Al Azad, Salauddin 1 ; Sun, Chihe 1 ; Zhang, Ezhen 2 ; Yan, Junshu 3 ; Samimi, Abdolreza 4 ; Sun, Fubao 1 ;
作者机构： 1.Jiangnan Univ, Sch Biotechnol, Minist Educ, Key Lab Carbohydrate Chem & Biotechnol, Wuxi 214122, Peoples R China
2.Guangxi Acad Agr Sci, Inst Agroprod Proc Sci & Technol, Nanning 530007, Peoples R China
3.Jiangsu Acad Agr Sci, Inst Anim Sci, Nanjing 210014, Jiangsu, Peoples R China
4.Univ Sistan & Baluchestan, Fac Engn, Chem Engn Dept, Zahedan, Iran
关键词： Enzymatic hydrolysis; Surfactants; Cellulase stability; Cellulase secondary structure; Molecular interaction
期刊名称：BIORESOURCE TECHNOLOGY （ 影响因子：9.0； 五年影响因子：9.5 ）
ISSN： 0960-8524
年卷期： 2025 年 416 卷
页码：
收录情况： SCI
摘要： Mixed surfactants improve the enzymatic hydrolysis of lignocellulosic substrates by enhancing cellulase stability against heat, pH, shear, and air-liquid interface stress. Under conditions of multiple factorial stresses (50 degrees C, pH 4.8, 180 rpm, and 15.5 cm2 air-liquid interface), cellulase with ternary surfactants (Tween 60/Triton X-114/ CTAB, the molar ratio 14:5.5:1) retained 84 % of its activity after 48 h of incubation, representing 1.15 and 1.29 folds that of the cellulase activity with the single Tween 60 and with no surfactants, respectively. This is attributed to the fact that ternary surfactants possess better rheology modulation and air-liquid interface competitiveness. In addition, the computational approach demonstrated that the ternary surfactants were capable of forming stronger hydrophobic and hydrogen-bond interactions with cellulase enzymes, thus maintaining its secondary structure and preventing the detrimental alpha-helix to beta-sheet transformation known to compromise cellulase activity. This synergy offers valuable insights into surfactant-cellulase interactions and supports efficient enzymatic hydrolysis in biorefineries.
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