Enhanced enzymatic hydrolysis of lignocellulosic substrate with less-expensive formulation of homemade cellulase cocktails
文献类型： 外文期刊
作者： Liu, Qiangqiang 1 ; Sun, Chihe 1 ; Madadi, Meysam 1 ; Dou, Shaohua 2 ; Yan, Junshu 3 ; Huan, Hailin 3 ; Samimi, Abdolreza 4 ; Ashori, Alireza 5 ; Sun, Fubao 1 ;
作者机构： 1.Jiangnan Univ, Sch Biotechnol, Key Lab Ind Biotechnol, Minist Educ, Wuxi 214122, Peoples R China
2.Dalian Univ, Coll Life Hlth, Dalian 116622, Peoples R China
3.Jiangsu Acad Agr Sci, Inst Anim Sci, Nanjing 210014, Jiangsu, Peoples R China
4.Univ Sistan & Baluchestan, Fac Engn, Dept Chem Engn, Zahedan, Iran
5.Iranian Res Org Sci & Technol, Dept Chem Technol, Tehran, Iran
关键词： Lignocellulosic biomass; High-solid saccharification; Surfactants mixture; Accessory enzymes; Non-catalytic protein; Synergistic effect
期刊名称：INDUSTRIAL CROPS AND PRODUCTS （ 影响因子：5.6； 五年影响因子：5.7 ）
ISSN： 0926-6690
年卷期： 2024 年 219 卷
页码：
收录情况： SCI
摘要： This study focused on the efficient production of fermentable sugars from high-solids enzymatic hydrolysis of the lignocellulosic substrate, using homemade cellulase preparation (from China company) combined with surfactants, plant-derived proteins, and accessory enzymes. Results show that various non-ionic and ionic surfactants worked together very effectively, resulting in the 48-h glucose yield of enzymatic hydrolysis increasing by 21.4 %, compared with a single surfactant. The surfactant mixture was particularly competitive at a lower enzyme loading and higher solid content of the enzymatic hydrolysis. Many plant-derived proteins were found to be useful in enzymatic hydrolysis. With the optimized mixture of surfactants, plant-derived protein, and accessory enzymes, the homemade cellulase preparation produced 170 g/L of fermentable sugars (similar to 90 % of the yield) from the enzymatic hydrolysis (2 mg/g dried substrate, 20 % (w/v), 72 h), which was promisingly economic and close to the starchy sugar. The homemade cellulase preparation by formulation with various surfactants and plant-derived proteins supplied a feasible solution for the development of a cost-effective enzymatic hydrolysis process.
- 相关文献
作者其他论文 更多>>
-
Dual assistance of surfactants in glycerol organosolv pretreatment and enzymatic hydrolysis of lignocellulosic biomass for bioethanol production
作者：Song, Guojie;Sun, Chihe;Madadi, Meysam;Sun, Fubao;Dou, Shaohua;Yan, Junshu;Huan, Hailin;Aghbashlo, Mortaza;Tabatabaei, Meisam;Tabatabaei, Meisam;Ashori, Alireza
关键词：Sugarcane bagasse; Surfactant-assisted pretreatment; High-solid enzymatic hydrolysis; Cellulosic ethanol; Economic analysis
-
Recent developments in heterologous production of cellulases and xylanases using the Pichia pastoris expression system
作者：Ullah, Hayat;Madadi, Meysam;Hu, Yun;Sun, Fubao;Asadollahi, Mohammad Ali;Dou, Shaohua;Yan, Junshu;Huan, Hailin;Madadi, Meysam;Sun, Fubao
关键词：cellulase; CRISPR/Cas9; genetic engineering strategies; P. pastoris; AOX1 promoter; xylanase
-
Hydrophobic modification improves the delivery of cell-penetrating peptides to eliminate intracellular pathogens in animals
作者：Tang, Qi;Tan, Peng;Dai, Zhaolai;Jin, Junqi;Zhang, Xin;Zhang, Yucheng;Zhou, Chenlong;Yue, Zitian;Fu, Huiyang;Ma, Xi;Wang, Tao;Ding, Yakun;Yan, Junshu;Ma, Xi
关键词：Intracellular targeting; Cell penetrating peptide; Antimicrobial peptide; Intracellular pathogens; Action mechanism
-
Bacterial lipopolysaccharide with different administration routes affects intestinal mucosal morphological, immunological, and microbial barrier functions in goslings
作者：Xi, Yumeng;Li, Yue;Ying, Shijia;Yan, Junshu;Shi, Zhendan
关键词：gosling; lipopolysaccharide challenge; intestinal mucosal barrier function
-
The effects of dietary protein and fiber levels on growth performance, gout occurrence, intestinal microbial communities, and immunoregulation in the gut-kidney axis of goslings
作者：Xi, Yumeng;Li, Yue;Yan, Junshu;Shi, Zhendan;Huang, Yuanpi;Huang, Yunmao
关键词：crude fiber; protein; gosling gout; intestinal microbial communities; gut-kidney axis
-
Effects of Manure Removal Frequencies and Deodorants on Ammonia and GHG Concentrations in Livestock House
作者：Zhang, Xia;Li, Jian;Shao, Le;Huan, Hailin;Qin, Feng;Zhai, Pin;Yang, Jie;Pan, Xiaoqing;Zhang, Xia;Li, Jian;Shao, Le;Huan, Hailin;Qin, Feng;Zhai, Pin;Yang, Jie;Pan, Xiaoqing
关键词：ammonia; greenhouse gas; manure remove frequency; microbial deodorant; VenaZn deodorant
-
Effects of resveratrol and its derivative pterostilbene on hepatic injury and immunological stress of weaned piglets challenged with lipopolysaccharide
作者：Li, Yue;Tu, Feng;Cao, Jing;Yan, Junshu;Li, Yue;Zhang, Hao;Chen, Yanan;Hou, Xiang
关键词：hepatic injury; immunological stress; lipopolysaccharide; piglet; pterostilbene; resveratrol
