Analysis of the Carry-Over of Ochratoxin A from Feed to Milk, Blood, Urine, and Different Tissues of Dairy Cows Based on the Establishment of a Reliable LC-MS/MS Method
文献类型： 外文期刊
作者： Zhang, Zhiqi 1 ; Fan, Zhichen 1 ; Nie, Dongxia 1 ; Zhao, Zhihui 1 ; Han, Zheng 1 ;
作者机构： 1.Shanghai Acad Agr Sci, Shanghai Key Lab Protected Hort Technol, Inst Agrofood Stand & Testing Technol, Shanghai 201403, Peoples R China
关键词： LC-MS; MS; dairy cows; ochratoxin A; ochratoxin alpha; carry-over
期刊名称：MOLECULES （ 影响因子：4.411； 五年影响因子：4.587 ）
ISSN：
年卷期： 2019 年 24 卷 15 期
页码：
收录情况： SCI
摘要： A rapid and reliable liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for simultaneous determination of ochratoxin A (OTA) and its metabolite ochratoxin alpha (OT alpha), for the first time, in dairy cow plasma, milk, urine, heart, liver, spleen, lung, and kidney. The established method was extensively validated by determining the linearity (R-2 >= 0.990), sensitivity (lower limit of quantification, 0.1-0.2 ng mL(-1)), recovery (75.3-114.1%), precision (RSD <= 13.6%), and stability (>= 83.0%). Based on the methodological advances, the carry-over of OTA was subsequently studied after oral administration of 30 mu g/kg body weight OTA to dairy cows. As revealed, OTA and OT alpha were detected in urine, with maximal concentrations of 1.8 ng mL(-1) and 324.6 ng mL(-1), respectively, but not in milk, plasma, or different tissues, verifying the protection effects of rumen flora against OTA exposure for dairy cows. Moreover, 100 fresh milk samples randomly collected from different supermarkets in Shanghai were also analyzed, and no positive samples were found, further proving the correctness of the in vivo biotransformation results. Thus, from the currently available data, regarding OTA contamination issues on dairy cows, no significant health risks were related to OTA exposure due to the consumption of these products.
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