Identification and characterization of two high affinity aptamers specific for Salmonella Enteritidis
文献类型： 外文期刊
作者： Zhang, Zilei 1 ; Liu, Danlei 1 ; Bai, Yalong 2 ; Cui, Yan 1 ; Wang, Dapeng 1 ; Shi, Xianming 1 ;
作者机构： 1.Shanghai Jiao Tong Univ, Sch Agr & Biol, MOST USDA Joint Res Ctr Food Safety, State Key Lab Microbial Metab, Shanghai 200240, Peoples R China
2.Shanghai Acad Agr Sci, Minist Agr, Inst Agrofood Stand & Testing Technol, Lab Qual & Safety Risk Assessment Agroprod Shangh, Shanghai 200240, Peoples R China
关键词： Aptamer; Salmonella enteritidis; Binding affinity; Next generation sequencing; Aptasensor; Detection
期刊名称：FOOD CONTROL （ 影响因子：5.548； 五年影响因子：5.498 ）
ISSN： 0956-7135
年卷期： 2019 年 106 卷
页码：
收录情况： SCI
摘要： DNA aptamers are promising candidates for bio-recognition assays due to their accumulation of bacterial cells. This feature can be used to identify specific pathogens in food samples. However, the conventional process of aptamer screening by randomly picking bacterial plate colonies for Sanger sequencing risks the elimination of superior sequences. In this study, we screened and evaluated all aptamers that were targeted to Salmonella Enteritidis (S. Enteritidis). After 13 rounds of selection we obtained 77,296 unique sequences from a total of 3,225,931 sequences using Next Generation Sequencing. We identified two high affinity aptamers that possessed dissociation constants of 43.8 and 56.2 nM. In addition, these two aptamers showed low binding activity to other foodbome bacterial isolates including Staphylococcus aureus, Listeria monocytogenes and Escherichia coli. Furthermore, by incorporation of AuNP-based colorimetric aptasensor, both aptamers showed alterations in color in proportion to the number of S. Enteritidis cells in the range of 10(4) to 10(5) copies. These aptamers are promising ligands for the specific recognition and capture of S. Enteritidis from clinical, environmental and food samples.
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