EGFR promotes the proliferation of quail follicular granulosa cells through the MAPK/extracellular signal-regulated kinase (ERK) signaling pathway
文献类型： 外文期刊
作者： Wu, Yan 1 ; Xiao, Hongwei 1 ; Pi, Jinsong 1 ; Zhang, Hao 1 ; Pan, Ailuan 1 ; Pu, Yuejin 1 ; Liang, Zhenhua 1 ; Shen, Jie; 1 ;
作者机构： 1.Hubei Acad Agr Sci, Inst Anim Husb & Vet, Wuhan, Hubei, Peoples R China
2.Key Lab Anim Embryo Engn & Mol Breeding Hubei Pro, Wuhan, Hubei, Peoples R China
关键词： Quail; follicular granulosa cells; follicle development; EGFR; MAPK; ERK
期刊名称：CELL CYCLE （ 影响因子：4.534； 五年影响因子：4.492 ）
ISSN： 1538-4101
年卷期：
页码：
收录情况： SCI
摘要： Follicles develop into preovulatory follicles during folliculogenesis and the majority of small yellow follicles become atretic and gets reabsorbed. In this study, based the RNA-seq results of duck ovary, epidermal growth factor receptor (EGFR) was selected as a candidate gene in follicular development and the role was explored. The results demonstrated that EGFR-P8 was the quail EGFR core promoter. It had an E2F4 binding site within EGFR core promoter. E2F4 overexpression significantly increased EGFR expression in quail granulosa cells (GCs). However, the effect was abolished when the GCs were treated with corynoxeine, an inhibitor of the mitogen-activated protein kinase/extracellular regulated protein kinase (MAPK/ERK) signaling pathway. Moreover, luciferase reporter assay and chromatin immunoprecipitation experiments showed that E2F4 upregulated the expression of EGFR expression, which increased E2 and P4 production. In addition, EGFR regulated GCs proliferation and affected follicular development. Taken together, our findings suggested that EGFR, which was regulated by E2F4, enhanced the expression of MAPK/ERK pathway components and follicular development. These results provided an important basis for an improved understanding of the MAPK/ERK pathway and new insight into the development of quail follicles.
- 相关文献
作者其他论文 更多>>
-
MiR-106a-5p by Targeting MAP3K2 Promotes Repair of Oxidative Stress Damage to the Intestinal Barrier in Prelaying Ducks
作者：Zhang, Li;Luo, Xiang;Tang, Rui;Wu, Yan;Liang, Zhenhua;Pi, Jinsong;Zhang, Hao;Zhang, Li;Luo, Xiang;Tang, Rui;Liu, Jingbo
关键词：apla-miR-106a-5p; intestinal barrier function; oxidative stress; prelaying ducks
-
Differential analysis of ovarian tissue between high and low-yielded laying hens in the late laying stage and the effect of LECT2 gene on follicular granulosa cells proliferation
作者：Chen, Lin;Li, Xianqiang;Wu, Yan;Pi, Jinsong;Chen, Lin;Li, Xianqiang;Wang, Jiaxiang
关键词：Laying hens; Laying performance; Oxidative stress; RNA-seq; LECT2 gene
-
High temperature curing softening salted egg yolk: Insights on microstructure, texture properties and composition of key protein
作者：Sun, Jing;Wu, Yan;Zeng, Qi;Liu, Wenhao;Du, Jinping;Pi, Jinsong;Lu, Lizhi;Zeng, Tao;Harlina, Putri Widyanti;Yang, Fang
关键词：Proteomics; Lipoprotein; Rheological property; Aggregation; Protein oxidation
-
Deciphering the carbon and nitrogen component conversion in humification process mediated by distinct microbial mechanisms in composting from different domestic organic wastes
作者：Zhan, Yabin;Chen, Yunfeng;Yang, Li;Liu, Bo;Zhan, Yabin;Li, Ruoqi;Chen, Wenjie;Chen, Yunfeng;Yang, Li;Liu, Bo;Li, Jun;Chang, Yuan;Wei, Yuquan;Li, Ji;Zhan, Yabin;Li, Ruoqi;Chen, Wenjie;Tao, Xingling;Li, Jun;Chang, Yuan;Wei, Yuquan;Li, Ji;Chen, Peizhen;Wang, Zhigang;Zhang, Hao
关键词：Food waste; Vegetable waste; Composting; Humic acid; Microbial community
-
Genome-Wide Association Study of Egg Production Traits in Shuanglian Chickens Using Whole Genome Sequencing
作者：Fu, Ming;Wu, Yan;Shen, Jie;Pan, Ailuan;Zhang, Hao;Sun, Jing;Liang, Zhenhua;Huang, Tao;Du, Jinping;Pi, Jinsong;Fu, Ming;Wu, Yan;Shen, Jie;Pan, Ailuan;Zhang, Hao;Sun, Jing;Liang, Zhenhua;Huang, Tao;Du, Jinping;Pi, Jinsong
关键词：Shuanglian chickens; egg production trait; GWAS; whole genome sequencing
-
Recent Advances in the Genetic and Biochemical Mechanisms of Rice Resistance to Brown Planthoppers (Nilaparvata lugens Stal)
作者：Shi, Shaojie;Wang, Huiying;Zha, Wenjun;Wu, Yan;Liu, Kai;Xu, Deze;Zhou, Lei;You, Aiqing;He, Guangcun;Zhou, Lei;You, Aiqing
关键词：rice; brown planthopper; defense responses; BPH-resistance genes; integrated pest management
-
The Role and Mechanism of Retinol and Its Transformation Product, Retinoic Acid, in Modulating Oxidative Stress-Induced Damage to the Duck Intestinal Epithelial Barrier In Vitro
作者：Zhang, Li;Tang, Rui;Wu, Yan;Liang, Zhenhua;Pi, Jinsong;Zhang, Hao;Zhang, Li;Tang, Rui;Liu, Jingbo;Zhang, Hao
关键词：retinol; retinoic acid; duck intestinal epithelial cell; oxidative stress; mechanism
