High-affinity phage-displayed peptide as a recognition probe for the detection of Cry2Ad2-3
文献类型： 外文期刊
作者： Wang, Yun 1 ; Zhang, Xiao 2 ; Xie, Yajing 2 ; Wu, Aihua 2 ; Zai, Xueming 1 ; Liu, Xianjin 2 ;
作者机构： 1.Jinling Inst Technol, Coll Hort, Nanjing 210038, Jiangsu, Peoples R China
2.Jiangsu Acad Agr Sci, Minist Sci & Technol, State Key Lab Cultivat Base, Jiangsu Key Lab Food Qual & Safety, Nanjing 210014, Jiangsu, Peoples R China
关键词： Cry2Ad2-3; BBMV-peptide Sandwich ELISA; Molecular docking
期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：6.953； 五年影响因子：6.737 ）
ISSN： 0141-8130
年卷期： 2019 年 137 卷
页码：
收录情况： SCI
摘要： Cry2A is widely used in transgenic crops in combination with Cry1 A toxins. The sensitive and robust detection of Cry2A toxin in food and the environment is necessary to monitor the safety of biopesticides. Here, we describe an approach that involves the use of phage-displayed peptide for the detection of Cry2Ad2-3-the main area of Cry2Ad2 insecticidal activity. After four rounds of panning, six positive monoclonal phage particles were obtained. Pep5 with a sequence of ACSYNHNSKCGGG displayed low cross-reactivity with other Cry toxins. The working range of detection for Cry2Ad2-3 toxin standards in the brush border membrane vesicle (BBMV)-peptide sandwich EUSA was 10-50.625 ng mL(-1) and the detection limit (LOD) was 8 ng mL(-1). Molecular insight into the interaction of Pep5 with Cry2Ad2-3 was gleaned using homology modeling and docking. Molecular docking results showed that high-affinity peptide tended to dock in the groove between the two domains of Cry2Ad2-3. The interactions within the toxin-pep5 complex were due to hydrogen bond and hydrophobic interaction. Pep5 also lead us to trap the binding region. Therefore, peptides may be a cost-efficient alternative for detecting Cry toxins and studying their mechanisms. (C) 2019 Elsevier B.V. All rights reserved.
- 相关文献
作者其他论文 更多>>
-
Combined detection of foodborne pathogens in irrigation water in Jiangsu, China
作者：Xie, Yajing;Zhang, Cunzheng;Liu, Xianjin;Xie, Yajing;Lu, Jiayao;Liu, Xianjin;Li, Bin;Fan, Bochao;Li, Dandi;Du, Xuefei;Xie, Yajing;Schmidt, Stefan
关键词：Irrigation water; Foodborne pathogens; Combined detection; Noroviruses; Strawberry production
-
A fast and efficient virtual screening and identification strategy for helix peptide binders based on finDr webserver: A case study of bovine serum albumin (BSA)
作者：Bu, Jiarui;Luo, Na;Liu, Yuan;Luo, Chuping;Zhang, Xiao;Bu, Jiarui;Luo, Na;Shen, Cheng;Xu, Chongxin;Zhu, Qing;Chen, Chengyu;Xie, Yajing;Liu, Xianjin;Liu, Yuan;Zhang, Xiao
关键词：Virtual screening; Affinity identification; Molecular simulations
-
An anti-idiotypic single domain antibody as Cry2Aa toxin mimotope and analysis of its binding region through molecular docking
作者：Lin, Manman;Han, Guangjie;Li, Chuanming;Huang, Lixin;Liu, Qin;Zhang, Nan;Xia, Yang;Lu, Yurong;Xu, Jian;Liu, Xianjin;Liu, Yuan;Lin, Johnson
关键词：Cry2Aa toxin; Anti-idiotypic antibody; Single-domain antibody; Epitopes; Mimotope; Molecular docking
-
Effects of neuropeptide F signaling on feeding, growth and development of Plutella xylostella (L.) larvae
作者：Shen, Cheng;Wu, Jiayi;Huang, Ziyan;He, Meng;Chen, Wei;Ilyas, Naila;Zhang, Xiao;Chen, Chengyu;Xu, Chongxin;Xie, Yajing;Liu, Yuan;Liu, Xianjin;Zhu, Qing;Shen, Cheng;Huang, Ziyan;Liu, Yuan;Liu, Xianjin;Wu, Jiayi;He, Meng;Xu, Chongxin;Xie, Yajing;Liu, Yuan;Liu, Xianjin;Zhu, Qing;Wang, Yun
关键词：Plutella xylostella; Neuropeptide F signaling; Feeding regulation
-
Generation of anti-idiotypic antibodies mimicking Cry2Aa toxin from an immunized mouse phage display library as potential insecticidal agents against Plutella xylostella
作者：Lin, Manman;Liu, Yuan;Zhang, Xiao;Xu, Chongxin;Hu, Xiaodan;Zhu, Qing;Xie, Yajing;Chen, Wei;Liu, Xianjin;Lin, Manman;Hu, Xiaodan;Xie, Yajing;Lin, Johnson;Liu, Yuan;Meng, Meng;Jin, Jiafeng;Shen, Cheng
关键词：Anti-idiotypic antibody; Single-chain variable fragment; Cry2Aa toxin; Insecticidal protein; Plutella xylostella
-
Enhanced composite Co-MOF-derived sodium carboxymethyl cellulose visual films for real-time and in situ monitoring fresh-cut apple freshness
作者：Zhang, Zhepeng;Zhang, Yang;Wang, Chen;Zou, Xiaobo;Guo, Zhiming;Liu, Xianjin;El-Seedi, Hesham R.;Gomez, Paula L.;Alzamora, Stella M.
关键词：Metal-organic framework; Multifunctional nano-filler; Sodium carboxymethyl cellulose; Intelligent film; Freshness monitoring
-
Cross-Reactivity of Human, Wild Boar, and Farm Animal Sera from Pre- and Post-Pandemic Periods with Alpha- and Βeta-Coronaviruses (CoV), including SARS-CoV-2
作者：Hulst, Marcel;Kant, Arie;Harders-Westerveen, Jose;Van der Poel, Wim H. M.;Hoffmann, Markus;Hoffmann, Markus;Xie, Yajing;Laheij, Charlotte;Murk, Jean-Luc
关键词：SARS-CoV-2; alpha- and beta-coronaviruses; cross-neutralization; pre- and post-pandemic sera; humans; farm animals; wild boars
