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Development of 28 EST-SSR markers based on transcriptome sequences of Gobiobotia filifer and cross-species amplification

文献类型： 外文期刊

作者： Dong, Weiwei 1 ; Tian, Huiwu 1 ; Wang, Dengqiang 1 ; Yu, Lixiong 1 ; Duan, Xinbin 1 ; Liu, Shaoping 1 ; Chen, Daqing 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan 430223, Hubei, Peoples R China

2.Southwest Univ, Key Lab Freshwater Fish Reprod & Dev, Minist Educ, Key Lab Aquat Sci Chongqing,Sch Life Sci, Chongqing, Peoples R China

关键词： cross-species; EST-SSR markers; G; filifer; transcriptome

期刊名称：JOURNAL OF APPLIED ICHTHYOLOGY （ 影响因子：0.892； 五年影响因子：0.956 ）

ISSN： 0175-8659

年卷期：

页码：

收录情况： SCI

摘要： Gobiobotia filifer is a small benthic fish distributed in Yangtze River Basin. The abundance of G. filifer increased after impoundment of Xiluodu Dam and Xiangjiaba Dam. The state of population structure and changes of genetic diversity before and after impoundment of Xiluodu Dam and Xiangjiaba Dam were interesting issues. However, efficient molecular markers were rare, which will limit us to solve above problems. Twenty-eight expressed sequence tag SSRs (EST-SSRs) were successfully identified and verified as stable amplification and polymorphic loci by polyacrylamide gel electrophoresis (PAGE) and capillary electrophoresis. The number of alleles at these EST-SSR loci ranged from 3 to 14, the polymorphism information content values were 0.125-0.897, and the observed and expected heterozygosities were 0.0-0.857 and 0.132-0.928, respectively. Cross-species amplification of the 28 loci developed in this study was examined in seven individuals of each of the 7 taxa. The amplification efficiency of 28 EST-SSRs primer pairs is related to the distance of genetic relationship between cross-species with G. filifer, and same subfamily species (Xenophysogobio boulengeri and Xenophysogobio nudicorpa) showed the highest (50%) amplification efficiency. These EST-SSR markers could be used to analyse genetic diversity and population structure of G. filifer and related species.

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