Cloning and characterization of the lignin biosynthesis genes NcCSE and NcHCT from Neolamarckia cadamba
文献类型： 外文期刊
作者： Li, Juncheng 1 ; Huang, Xiaoling 1 ; Huang, Hao 5 ; Huo, Heqiang 6 ; Nguyen, Chi D. 6 ; Pian, Ruiqi 1 ; Li, Huaqiang 7 ;
作者机构： 1.South China Agr Univ, Guangdong Key Lab Innovat Dev & Utilizat ForestPl, Wushan Rd 483, Guangzhou 510642, Guangdong, Peoples R China
2.Guangdong Acad Agr Sci, Inst Fruit Tree Res, Guangdong Key Lab Trop & Subtrop Fruit Tree Res, Guangzhou 510640, Guangdong, Peoples R China
3.South China Agr Univ, Coll Forestry & Landscape Architecture, Guangdong Prov Res Ctr Woody Forage Engn Technol, Guangzhou 510642, Guangdong, Peoples R China
4.State Key Lab Conservat & Utilizat Subtrop Agrobi, Guangzhou, Guangdong, Peoples R China
5.Guangxi Bot Garden Med Plants, Nanning 530023, Peoples R China
6.Univ Florida, Inst Food & Agr Sci, Midflorida Res & Educ Ctr, Apopka, FL USA
7.State Run Leizhou Forestry Bur, Zhanjiang 524348, Guangdong, Peoples R China
关键词： Lignin; NcCSE; NcHCT; Neolamarckia cadamba; DEGs
期刊名称：AMB EXPRESS （ 影响因子：3.298； 五年影响因子：3.427 ）
ISSN： 2191-0855
年卷期： 2019 年 9 卷 1 期
页码：
收录情况： SCI
摘要： Neolamarckia cadamba is an important fast growing tree species used for pulping and wood material in industry for it's desirable wood properties. As one of the most important content in wood, lignin provides structural integrity, strength, and hydrophobicity to the thickened cell walls and is the major factor contributing to biomass recalcitrance. It does not reduce the palatability of forage grass for animals, but it hinders the isolation of cellulose fibers and the efficient enzymatic depolymerization of cellulose and hemicellulose into fermentable sugars in biorefining processes by limiting the access by hydrolytic enzymes to their polysaccharide substrates. This work focused on analyzing the functions of NcCSE (Caffeoyl Shikimate Esterase, GenBank accession number: MG739672) and NcHCT (Hydroxycinnamoyl Transferase,GenBank accession number: MG739673) in the lignin biosynthetic process in order to improve the potential for utilization of leaves and wood from N. cadamba. The mutant phenotype of cse-2 was dramatically complemented to WT in the stable transgenic lines cse-35S::NcCSE, but overexpression of NcHCT in the cse-2 mutant did not have the same result as cse-35S::NcCSE, providing only partial complementation.
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